ArticleAmerican journal of physiology. Cell physiology2013
A novel in vitro model system for smooth muscle differentiation from human embryonic stem cell-derived mesenchymal cells.
Article in American journal of physiology. Cell physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 34 citations in OpenAlex.
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- PD-L1 mediates lung fibroblast to myofibroblast transition through Smad3 and β-catenin signaling pathways.Scientific reports · 2022Article
- Dedicator of Cytokinesis 2 (DOCK2) Deficiency Attenuates Lung Injury Associated with Chronic High-Fat and High-Fructose Diet-Induced Obesity.The American journal of pathology · 2022Article
- Growth differentiation factor 11 promotes differentiation of MSCs into endothelial-like cells for angiogenesis.Journal of cellular and molecular medicine · 2020Article
- Response of vascular mesenchymal stem/progenitor cells to hyperlipidemia.Cellular and molecular life sciences : CMLS · 2018Review
- Stem Cell Sources and Graft Material for Vascular Tissue Engineering.Stem cell reviews and reports · 2018Review
- DOCK2 deficiency mitigates HFD-induced obesity by reducing adipose tissue inflammation and increasing energy expenditure.Journal of lipid research · 2017Article
- Isolation and characterization of equine endometrial mesenchymal stromal cells.Stem cell research & therapy · 2017Article
- ADAR1-Mediated RNA Editing, A Novel Mechanism Controlling Phenotypic Modulation of Vascular Smooth Muscle Cells.Circulation research · 2016Article
- RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix.Nature communications · 2016Article
- Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering.Stem cells international · 2016Article
- Dedicator of cytokinesis 2, a novel regulator for smooth muscle phenotypic modulation and vascular remodeling.Circulation research · 2015Article
- 3D functional and perfusable microvascular networks for organotypic microfluidic models.Journal of materials science. Materials in medicine · 2015Review
- Olfactomedin 2, a novel regulator for transforming growth factor-β-induced smooth muscle differentiation of human embryonic stem cell-derived mesenchymal cells.Molecular biology of the cell · 2014Article
- Endothelial cells direct mesenchymal stem cells toward a smooth muscle cell fate.Stem cells and development · 2014Article
- Adult vascular smooth muscle cells in culture express neural stem cell markers typical of resident multipotent vascular stem cells.Cell and tissue research · 2014Article
- Generation of 3D functional microvascular networks with human mesenchymal stem cells in microfluidic systems.Integrative biology : quantitative biosciences from nano to macro · 2014Article
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4 authors at 2 institutions in 1 country.
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Abstract
The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated to SMCs by transforming growth factor-β (TGF-β) in a dose- and time-dependent manner as demonstrated by the expression of SMC-specific genes smooth muscle α-actin, calponin, and smooth muscle myosin heavy chain. Under normal growth conditions, however, the differentiation capacity of hES-MCs was very limited. hES-MC-derived SMCs had an elongated and spindle-shaped morphology and contracted in response to the induction of carbachol and KCl. KCl-induced calcium transient was also evident in these cells. Compared with the parental cells, TGF-β-treated hES-MCs sustained the endothelial tube formation for a longer time due to the sustained SMC phenotype. Mechanistically, TGF-β-induced differentiation was both Smad- and serum response factor/myocardin dependent. TGF-β regulated myocardin expression via multiple signaling pathways including Smad2/3, p38 MAPK, and PI3K. Importantly, we found that a low level of myocardin was present in mesoderm prior to SMC lineage determination, and a high level of myocardin was not induced until the differentiation process was initiated. Taken together, our study characterized a novel SMC differentiation model that can be used for studying human SMC differentiation from mesoderm during vascular development.
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