ArticleStructure (London, England : 1993)2012
Protein secondary structure determination by constrained single-particle cryo-electron tomography.
Article in Structure (London, England : 1993), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Article
- In situ structure determination of conformationally flexible targets using nextPYP.Nature protocols · 2026Review
- Strategies for studying discrete heterogeneity in situ using cryo-electron tomography.Current opinion in structural biology · 2025Review
- An image processing pipeline for electron cryo-tomography in RELION-5.FEBS open bio · 2024Article
- CryoDRGN-ET: deep reconstructing generative networks for visualizing dynamic biomolecules inside cells.Nature methods · 2024Article
- Learning structural heterogeneity from cryo-electron sub-tomograms with tomoDRGN.Nature methods · 2024Article
- Advances in cryo-ET data processing: meeting the demands of visual proteomics.Current opinion in structural biology · 2024Review
- Accurate, automatic determination of astigmatism and phase with Ctfplotter in IMOD.Journal of structural biology · 2024Article
- The big chill: Growth ofQRB discovery · 2024Review
- nextPYP: a comprehensive and scalable platform for characterizing protein variability in situ using single-particle cryo-electron tomography.Nature methods · 2023Article
- Molecular architecture and conservation of an immature human endogenous retrovirus.Nature communications · 2023Article
- Molecular architecture and conservation of an immature human endogenous retrovirus.bioRxiv : the preprint server for biology · 2023Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- Article
- High-resolution structure determination using high-throughput electron cryo-tomography.Acta crystallographica. Section D, Structural biology · 2022Review
- Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells.Journal of structural biology · 2022Article
- Cryogenic electron microscopy approaches that combine images and tilt series.Microscopy (Oxford, England) · 2022Article
- In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM.Current opinion in structural biology · 2022Review
- Article
- Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
Cryo-electron microscopy (cryo-EM) is a powerful technique for 3D structure determination of protein complexes by averaging information from individual molecular images. The resolutions that can be achieved with single-particle cryo-EM are frequently limited by inaccuracies in assigning molecular orientations based solely on 2D projection images. Tomographic data collection schemes, however, provide powerful constraints that can be used to more accurately determine molecular orientations necessary for 3D reconstruction. Here, we propose "constrained single-particle tomography" as a general strategy for 3D structure determination in cryo-EM. A key component of our approach is the effective use of images recorded in tilt series to extract high-resolution information and correct for the contrast transfer function. By incorporating geometric constraints into the refinement to improve orientational accuracy of images, we reduce model bias and overrefinement artifacts and demonstrate that protein structures can be determined at resolutions of ∼8 Å starting from low-dose tomographic tilt series.
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Registered trials
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