ArticleBMC infectious diseases2012
Induction of IFNα or IL-12 depends on differentiation of THP-1 cells in dengue infections without and with antibody enhancement.
Article in BMC infectious diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
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- Immunopathogenesis of Different Emerging Viral Infections: Evasion, Fatal Mechanism, and Prevention.Frontiers in immunology · 2021Review
- Impaired production of immune mediators in dengue virus type 2-infected mononuclear cells of adults with end stage renal disease.Scientific reports · 2019Article
- Detection of post-vaccination enhanced dengue virus infection in macaques: An improved model for early assessment of dengue vaccines.PLoS pathogens · 2019Article
- Induction of tryptophan 2,3-dioxygenase expression in human monocytic leukemia/lymphoma cell lines THP-1 and U937.International journal of tryptophan research : IJTR · 2019Article
- The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages.The Journal of biological chemistry · 2018Article
- Cellular FLIP long isoform (cFLIPThe Journal of biological chemistry · 2018Article
- A relevant in vitro human model for the study of Zika virus antibody-dependent enhancement.The Journal of general virology · 2017Article
- Sialylation and desialylation dynamics of monocytes upon differentiation and polarization to macrophages.Glycoconjugate journal · 2016Article
- Fc receptors in antibody-dependent enhancement of viral infections.Immunological reviews · 2015Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAppropriate induction of the early Th1 cytokine IL-12 is a critical defense directed against viral infection. We have previously shown that different viruses elicited either IL-12 or IFNα dependent Th1 reactions. Using dengue-2 virus, we sought to explore how dengue-2 induced IL-12 or IFNα expression by monocytic and its derived dendritic cells.
methodsWe employed human monocytic cell line, THP-1, to investigate whether differentiation of monocytic cells is involved in the switch between IFNα and IL-12 induction. Flow cytometry, RT-PCR and ELISA were respectively used to determine cell differentiation, IL-12 and IFNα mRNA expression and protein production.
resultsTHP-1, expressing CD123, which is a plasmacytoid dendritic cell marker, but not CD14, CD11b or CD11c revealed IFNα mRNA expression while stimulated by dengue-2. In contrast, PMA-induced THP-1 differentiation toward monocytic cells expressed CD11b+, and CD14+, but not CD123, and revealed exclusively IL-12 expression while stimulated by dengue-2. Further studies showed that CD123+ expressing THP-1 cells elicited higher IFNα expression in dose and time dependent induction after infection, and PMA-induced monocytic differentiation of THP-1 cells revealed IL-12 expression. Antibody-dependent enhancement of DEN-2 infection significantly suppressed the DEN-2 induced IL-12 p40 expression in monocytic differentiated THP-1 cells.
conclusionsClarification and modulation of the early Th1 reaction in different monocytic cells may change or prevent complication from dengue infection.
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