ArticleMolecular microbiology2013
Random mutagenesis and topology analysis of the autoinducing peptide biosynthesis proteins in Staphylococcus aureus.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- Hijacking the signal: a critical evaluation of quorum sensing inhibitors as a next-generation approach against Staphylococcus aureus.Cell communication and signaling : CCS · 2026Review
- Determinants of maturation of theJournal of bacteriology · 2024Article
- The role of Staphylococcus aureus quorum sensing in cutaneous and systemic infections.Inflammation and regeneration · 2024Review
- Quorum-sensing, intra- and inter-species competition in the staphylococci.Microbiology (Reading, England) · 2023Review
- Chemical and biomolecular insights into theIsrael journal of chemistry · 2023Article
- Conformational analysis and interaction of theFrontiers in chemistry · 2023Article
- Autoinducing peptide-based quorum signaling systems in Clostridioides difficile.Current opinion in microbiology · 2022Review
- Peptidomimetics as Potential Anti-Virulence Drugs Against Resistant Bacterial Pathogens.Frontiers in microbiology · 2022Review
- Interspecies Regulation BetweenFrontiers in microbiology · 2022Article
- Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia.Pathogens (Basel, Switzerland) · 2021Article
- Pathogenic Effect ofFrontiers in microbiology · 2020Article
- Article
- Regulation ofMicrobiology spectrum · 2019Review
- MroQ Is a Novel Abi-Domain Protein That Influences Virulence Gene Expression inInfection and immunity · 2019Article
- Bacterial sensing: A putative amphipathic helix in RsiV is the switch for activating σV in response to lysozyme.PLoS genetics · 2018Article
- Host Nitric Oxide Disrupts Microbial Cell-to-Cell Communication to Inhibit Staphylococcal Virulence.Cell host & microbe · 2018Article
- Coagulase-Negative Staphylococcal Strain Prevents Staphylococcus aureus Colonization and Skin Infection by Blocking Quorum Sensing.Cell host & microbe · 2017Article
- The SaeRS Two-Component System Controls Survival ofFrontiers in cellular and infection microbiology · 2017Article
- Impact of Environmental Cues on Staphylococcal Quorum Sensing and Biofilm Development.The Journal of biological chemistry · 2016Review
- Key driving forces in the biosynthesis of autoinducing peptides required for staphylococcal virulence.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Staphylococcus aureus accessory gene regulator (agr) is a peptide signalling system that regulates the production of secreted virulence factors required to cause infections. The signal controlling agr function is a 7-9 residue thiolactone-containing peptide called an autoinducing peptide (AIP) that is biosynthesized from the AgrD precursor by the membrane peptidase AgrB. To gain insight into AgrB and AgrD function, the agrBD genes were mutagenized and screened for deficiencies in AIP production. In total, single-site mutations at 14 different residues in AgrD were identified and another 20 within AgrB. In AgrD, novel mutations were characterized in the N-terminal leader and throughout the central region encoding the AIP signal. In AgrB, most mutations blocked peptidase activity, but mutations in the K129-K131 residues were defective in a later step in AIP biosynthesis, separating the peptidase function from thiolactone ring formation and AIP transport. With the identification of residues in AgrB essential for AgrD processing, we reevaluated the membrane topology and the new model predicts four transmembrane helices and a potential re-entrant loop on the cytoplasmic face. Finally, co-immunoprecipitation studies indicate that AgrB forms oligomeric structures within the membrane. These studies provide further insight into the unique structural and functional properties of AgrB.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.