ArticleJournal of the American Chemical Society2012
Using distal-site mutations and allosteric inhibition to tune, extend, and narrow the useful dynamic range of aptamer-based sensors.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- A robust Au-C[triple bond, length as m-dash]C anchoring group greatly improves the signal stability of electrochemical aptamer-based sensors forChemical science · 2026Article
- Advancements and Prospects in DNA-Based Bioanalytical Technology for Environmental Toxicant Detection.JACS Au · 2025Review
- Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.Nature nanotechnology · 2025Article
- Wearable Electrochemical Biosensors for Advanced Healthcare Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- DNA-controlled protein fluorescence: Design of aptamer-split peptide hetero-modulator for GFP to respond to intracellular ATP levels.Nucleic acids research · 2024Article
- Nucleic acid-based wearable and implantable electrochemical sensors.Chemical Society reviews · 2024Review
- Wearing the Lab: Advances and Challenges in Skin-Interfaced Systems for Continuous Biochemical Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Article
- Programmable self-regulated molecular buffers for precise sustained drug delivery.Nature communications · 2022Article
- The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays.Chemical science · 2022Article
- Critical Design Factors for Electrochemical Aptasensors Based on Target-Induced Conformational Changes: The Case of Small-Molecule Targets.Biosensors · 2022Review
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- Unraveling the effect of the aptamer complementary element on the performance of duplexed aptamers: a thermodynamic study.Analytical and bioanalytical chemistry · 2021Article
- A small-molecule chemical interface for molecular programs.Nucleic acids research · 2021Article
- Accelerating Post-SELEX Aptamer Engineering Using Exonuclease Digestion.Journal of the American Chemical Society · 2021Article
- Tuning Biosensor Cross-Reactivity Using Aptamer Mixtures.Analytical chemistry · 2020Article
- Graphene/aptamer probes for small molecule detection: from in vitro test to in situ imaging.Mikrochimica acta · 2020Review
- Perspective on the Future Role of Aptamers in Analytical Chemistry.Analytical chemistry · 2019Article
- Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation.Chemical science · 2019Article
- Increased Flexibility between Stems of Intramolecular Three-Way Junctions by the Insertion of Bulges.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Here we demonstrate multiple, complementary approaches by which to tune, extend, or narrow the dynamic range of aptamer-based sensors. Specifically, we employ both distal-site mutations and allosteric control to tune the affinity and dynamic range of a fluorescent aptamer beacon. We show that allosteric control, achieved by using a set of easily designed oligonucleotide inhibitors that competes against the folding of the aptamer, allows rational fine-tuning of the affinity of our model aptamer across 3 orders of magnitude of target concentration with greater precision than that achieved using mutational approaches. Using these methods, we generate sets of aptamers varying significantly in target affinity and then combine them to recreate several of the mechanisms employed by nature to narrow or broaden the dynamic range of biological receptors. Such ability to finely control the affinity and dynamic range of aptamers may find many applications in synthetic biology, drug delivery, and targeted therapies, fields in which aptamers are of rapidly growing importance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.