ArticleBiology open2012
Chromatin remodelling initiation during human spermiogenesis.
Article in Biology open, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Unravelling male infertility through epigenetics: present understanding and future challenges.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Review
- Of mice and men: translating mouse knockout models of human male infertility.Reproduction (Cambridge, England) · 2025Review
- Epigenetics of nonobstructive azoospermia.Asian journal of andrology · 2025Review
- Review
- Nucleosomes in mammalian sperm: conveying paternal epigenetic inheritance or subject to reprogramming between generations?Current opinion in genetics & development · 2023Review
- Functional association between telomeres, oxidation and mitochondria.Frontiers in reproductive health · 2023Review
- Global 5mC and 5hmC DNA Levels in Human Sperm Subpopulations with Differentially Protaminated Chromatin in Normo- and Oligoasthenozoospermic Males.International journal of molecular sciences · 2022Article
- Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids.Epigenetics & chromatin · 2021Article
- Histone Post-Translational Modifications and CircRNAs in Mouse and Human Spermatozoa: Potential Epigenetic Marks to Assess Human Sperm Quality.Journal of clinical medicine · 2020Review
- Telomere Dynamics Throughout Spermatogenesis.Genes · 2019Article
- Chromosome (re)positioning in spermatozoa of fathers and sons - carriers of reciprocal chromosome translocation (RCT).BMC medical genomics · 2019Article
- Altered three-dimensional organization of sperm genome in DPY19L2-deficient globozoospermic patients.Journal of assisted reproduction and genetics · 2019Article
- Essential Role of Histone Replacement and Modifications in Male Fertility.Frontiers in genetics · 2019Review
- RNA sequencing reveals candidate genes and polymorphisms related to sperm DNA integrity in testis tissue from boars.BMC veterinary research · 2017Article
- Global mapping of post-translational modifications on histone H3 variants in mouse testes.Biochemistry and biophysics reports · 2017Article
- Epigenetic reprogramming of the zygote in mice and men: on your marks, get set, go!Reproduction (Cambridge, England) · 2016Review
- Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer.Nature protocols · 2016Article
- Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures.Cell reports · 2015Article
- Genetic dosage and position effect of small supernumerary marker chromosome (sSMC) in human sperm nuclei in infertile male patient.Scientific reports · 2015Article
- Characterization of BRD4 during mammalian postmeiotic sperm development.Molecular and cellular biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the last phase of spermatogenesis, spermiogenesis, haploid round spermatids metamorphose towards spermatozoa. Extensive cytoplasmic reduction and chromatin remodelling together allow a dramatic decrease of cellular, notably nuclear volume. DNA packing by a nucleosome based chromatin structure is largely replaced by a protamine based one. At the cytoplasmic level among others the acrosome and perinuclear theca (PNT) are formed. In this study we describe the onset of chromatin remodelling to occur concomitantly with acrosome and PNT development. In spread human round spermatid nuclei, we show development of a DAPI-intense doughnut-like structure co-localizing with the acrosomal sac and sub acrosomal PNT. At this structure we observe the first gradual decrease of nucleosomes and several histones. Histone post-translational modifications linked to chromatin remodelling such as H4K8ac and H4K16ac also delineate the doughnut, that is furthermore marked by H3K9me2. During the capping phase of acrosome development, the size of the doughnut-like chromatin domain increases, and this area often is marked by uniform nucleosome loss and the first appearance of transition protein 2 and protamine 1. In the acrosome phase at nuclear elongation, chromatin remodelling follows the downward movement of the marginal ring of the acrosome. Our results indicate that acrosome development and chromatin remodelling are interacting processes. In the discussion we relate chromatin remodelling to the available data on the nuclear envelope and the linker of nucleoskeleton and cytoskeleton (LINC) complex of spermatids, suggesting a signalling route for triggering chromatin remodelling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.