ArticleBiology open2012
A role for Rap2 in recycling the extended conformation of LFA-1 during T cell migration.
Article in Biology open, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Liver transcriptome dynamics in Holstein cows during the periparturient transition.Scientific reports · 2026Article
- Ubiquitylation-dependent Rap2 activation regulates lamellipodia dynamics during cell migration.Journal of cell science · 2025Article
- Syndapin and GTPase RAP-1 control endocytic recycling via RHO-1 and non-muscle myosin II.Current biology : CB · 2023Article
- Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble.Scientific reports · 2022Article
- Redox Control of Integrin-Mediated Hepatic Inflammation in Systemic Autoimmunity.Antioxidants & redox signaling · 2022Review
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- The Rap2c GTPase facilitates B cell receptor-induced reorientation of the microtubule-organizing center.Small GTPases · 2020Article
- Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes.Biophysical journal · 2020Article
- Integrin α5β1 inhibition by ATN-161 reduces neuroinflammation and is neuroprotective in ischemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- RAP GTPases and platelet integrin signaling.Platelets · 2019Review
- Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes.Bio-protocol · 2018Article
- Ras-related protein Rap2c promotes the migration and invasion of human osteosarcoma cells.Oncology letters · 2018Article
- Integrins in T Cell Physiology.International journal of molecular sciences · 2018Review
- Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- LFA-1 in T Cell Migration and Differentiation.Frontiers in immunology · 2018Review
- Role of Mechanotransduction and Tension in T Cell Function.Frontiers in immunology · 2018Review
- A novel intracellular pool of LFA-1 is critical for asymmetric CD8The Journal of cell biology · 2017Article
- The role of small GTPases and EPAC-Rap signaling in the regulation of the blood-brain and blood-retinal barriers.Tissue barriers · 2017Review
- Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.The Journal of biological chemistry · 2016Article
- A novel multi-parameter assay to dissect the pharmacological effects of different modes of integrin αLβ2 inhibition in whole blood.British journal of pharmacology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
T lymphocytes make use of their major integrin LFA-1 to migrate on surfaces that express ICAM-1 such as blood vessels and inflamed tissue sites. How the adhesions are turned over in order to supply traction for this migration has not been extensively investigated. By following the fate of biotinylated membrane LFA-1 on T lymphocytes, we show in this study that LFA-1 internalization and re-exposure on the plasma membrane are linked to migration. Previously we demonstrated the GTPase Rap2 to be a regulator of LFA-1-mediated migration. SiRNA knockdown of this GTPase inhibits both LFA-1 internalization and also its ability to be re-exposed, indicating that Rap2 participates in recycling of LFA-1 and influences its complete endocytosis-exocytosis cycle. Confocal microscopy images reveal that the intracellular distribution of Rap2 overlaps with endosomal recycling vesicles. Although the homologous GTPase Rap1 is also found on intracellular vesicles and associated with LFA-1 activation, these two homologous GTPases do not co-localize. Little is known about the conformation of the LFA-1 that is recycled. We show that the extended form of LFA-1 is internalized and in Rap2 siRNA-treated T lymphocytes the trafficking of this LFA-1 conformation is disrupted resulting in its intracellular accumulation. Thus LFA-1-mediated migration of T lymphocytes requires Rap2-expressing vesicles to recycle the extended form of LFA-1 that we have previously found to control migration at the leading edge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.