ArticleInternational journal of plant genomics2012
Gel-based and gel-free quantitative proteomics approaches at a glance.
Article in International journal of plant genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses.Frontiers in genetics · 2022Pooled it
- Proteomic Profiles and Biological Processes of Relapsed vs. Non-Relapsed Pediatric Hodgkin Lymphoma.International journal of molecular sciences · 2020Trial
- Isolation of Cytoskeleton and Cytoskeleton-Bound Polysome, and Identification of Cytoskeletal Proteins from a Grain Legume.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Dynamic expression of the zebrafish (Danio rerio) proteome across early larval development.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2025Article
- Seed protein electrophoresis in plant genetics: Commemorating the pioneering contributions of Prof. Chittaranjan Kole and team to the foundation of plant proteomics.The plant genome · 2025Review
- Current methods and future needs for visible and non-visible detection of plant stress responses.Frontiers in plant science · 2025Review
- Deciphering Cancer Complexity: Integrative Proteogenomics and Proteomics Approaches for Biomarker Discovery.Methods in molecular biology (Clifton, N.J.) · 2025Review
- The cancer microbiome.Advances in clinical chemistry · 2025Review
- Proteomic Insights into Osteoporosis: Unraveling Diagnostic Markers of and Therapeutic Targets for the Metabolic Bone Disease.Biomolecules · 2024Review
- Exploring the secretome ofFrontiers in bioengineering and biotechnology · 2024Article
- Development and Optimization of Label-Free Quantitative Proteomics under Different Crossing Periods of Bottle Gourd.Current issues in molecular biology · 2023Article
- Prospects of microgreens as budding living functional food: Breeding and biofortification through OMICS and other approaches for nutritional security.Frontiers in genetics · 2023Review
- The roles of plant proteases and protease inhibitors in drought response: a review.Frontiers in plant science · 2023Review
- A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation.International journal of molecular sciences · 2022Article
- Proteomic and clinical biomarkers for acute mountain sickness in a longitudinal cohort.Communications biology · 2022Article
- Wheat Proteomics for Abiotic Stress Tolerance and Root System Architecture: Current Status and Future Prospects.Proteomes · 2022Review
- In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry.BMC plant biology · 2021Article
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- SWATH Based Quantitative Proteomics Reveals Significant Lipid Metabolism in Early Myopic Guinea Pig Retina.International journal of molecular sciences · 2021Article
- Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture.Proteome science · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-dimensional gel electrophoresis (2-DE) is widely applied and remains the method of choice in proteomics; however, pervasive 2-DE-related concerns undermine its prospects as a dominant separation technique in proteome research. Consequently, the state-of-the-art shotgun techniques are slowly taking over and utilising the rapid expansion and advancement of mass spectrometry (MS) to provide a new toolbox of gel-free quantitative techniques. When coupled to MS, the shotgun proteomic pipeline can fuel new routes in sensitive and high-throughput profiling of proteins, leading to a high accuracy in quantification. Although label-based approaches, either chemical or metabolic, gained popularity in quantitative proteomics because of the multiplexing capacity, these approaches are not without drawbacks. The burgeoning label-free methods are tag independent and suitable for all kinds of samples. The challenges in quantitative proteomics are more prominent in plants due to difficulties in protein extraction, some protein abundance in green tissue, and the absence of well-annotated and completed genome sequences. The goal of this perspective assay is to present the balance between the strengths and weaknesses of the available gel-based and -free methods and their application to plants. The latest trends in peptide fractionation amenable to MS analysis are as well discussed.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.