ArticleProceedings of the National Academy of Sciences of the United States of America2012
Complete fiber structures of complex trimeric autotransporter adhesins conserved in enterobacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Insights into the complex formation of a trimeric autotransporter adhesin with a peptidoglycan-binding periplasmic protein.Cell surface (Amsterdam, Netherlands) · 2025Article
- Chain formation mediated by Escherichia coli immunoglobulin-binding proteins EibD and EibG depends on expression levels and localization of proteins.Scientific reports · 2025Article
- Architectural dissection of adhesive bacterial cell surface appendages from a "molecular machines" viewpoint.Journal of bacteriology · 2024Review
- Virulence-linked adhesin drives mutualist colonization of the bee gut via biofilm formation.bioRxiv : the preprint server for biology · 2024Article
- The Trimeric Autotransporter Adhesin SadA fromVaccines · 2024Article
- Adhesion preference of the sticky bacteriumFrontiers in bioengineering and biotechnology · 2024Article
- A trimeric coiled-coil motif binds bacterial lipopolysaccharides with picomolar affinity.Frontiers in cellular and infection microbiology · 2023Article
- Adhesion of Bartonella henselae to Fibronectin Is Mediated via Repetitive Motifs Present in the Stalk ofMicrobiology spectrum · 2022Article
- The C-terminal head domain of Burkholderia pseudomallei BpaC has a striking hydrophilic core with an extensive solvent network.Molecular microbiology · 2022Article
- BamA and BamD Are Essential for the Secretion of Trimeric Autotransporter Adhesins.Frontiers in microbiology · 2021Article
- Coaggregation properties of trimeric autotransporter adhesins.MicrobiologyOpen · 2020Article
- Recent advances in the understanding of trimeric autotransporter adhesins.Medical microbiology and immunology · 2020Review
- Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets.Medical microbiology and immunology · 2020Review
- Non-adaptive Evolution of Trimeric Autotransporters inFrontiers in microbiology · 2020Article
- Structure of the UspA1 protein fragment from Moraxella catarrhalis responsible for C3d binding.Journal of structural biology · 2019Article
- Article
- Interactions between the Trimeric Autotransporter Adhesin EmaA and Collagen Revealed by Three-Dimensional Electron Tomography.Journal of bacteriology · 2019Article
- Unique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules.Nature communications · 2019Article
- Type V Secretion in Gram-Negative Bacteria.EcoSal Plus · 2019Article
- Folding of a bacterial integral outer membrane protein is initiated in the periplasm.Nature communications · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trimeric autotransporter adhesins (TAAs) are modular, highly repetitive surface proteins that mediate adhesion to host cells in a broad range of Gram-negative pathogens. Although their sizes may differ by more than one order of magnitude, they all follow the same basic head-stalk-anchor architecture, where the head mediates adhesion and autoagglutination, the stalk projects the head from the bacterial surface, and the anchor provides the export function and attaches the adhesin to the bacterial outer membrane after export is complete. In complex adhesins, head and stalk domains may alternate several times before the anchor is reached. Despite extensive sequence divergence, the structures of TAA domains are highly constrained, due to the tight interleaving of their constituent polypeptide chains. We have therefore taken a "domain dictionary" approach to characterize representatives for each domain type by X-ray crystallography and use these structures to reconstruct complete TAA fibers. With SadA from Salmonella enterica, EhaG from enteropathogenic Escherichia coli (EHEC), and UpaG from uropathogenic E. coli (UPEC), we present three representative structures of a complex adhesin that occur in a conserved genomic context in Enterobacteria and is essential in the infection process of uropathogenic E. coli. Our work proves the applicability of the dictionary approach to understanding the structure of a class of proteins that are otherwise poorly tractable by high-resolution methods and provides a basis for the rapid and detailed annotation of newly identified TAAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.