ArticleLasers in surgery and medicine2012
Topical rapamycin suppresses the angiogenesis pathways induced by pulsed dye laser: molecular mechanisms of inhibition of regeneration and revascularization of photocoagulated cutaneous blood vessels.
Article in Lasers in surgery and medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- The Diagnosis and Treatment Technology of Port-Wine Stain, and the Prospect of Future Treatment.Dermatology and therapy · 2026Review
- Applications of Graphene Family Nanomaterials in Regenerative Medicine: Recent Advances, Challenges, and Future Perspectives.International journal of nanomedicine · 2024Review
- Hemoporfin-Mediated Photodynamic Therapy for Port-Wine Stains on Extremities.Dermatology and therapy · 2023Article
- Intralesional Axitinib Injection Mitigates Hypertrophic Scar by Inhibiting Angiogenesis Pathway: A Preliminary Study in a Rabbit Ear Model.Clinical, cosmetic and investigational dermatology · 2023Article
- Pathogenesis of Port-Wine Stains: Directions for Future Therapies.International journal of molecular sciences · 2022Review
- Hemoporfin-Mediated Photodynamic Therapy for Port-Wine Stains: Multivariate Analysis of Clinical Efficacy and Optical Coherence Tomography Appearance.Frontiers in medicine · 2022Article
- Removing Subcutaneous Microvessels Using Photo-Mediated Ultrasound Therapy.Lasers in surgery and medicine · 2020Article
- Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels.Histology and histopathology · 2019Article
- Ponatinib Combined With Rapamycin Causes Regression of Murine Venous Malformation.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Arterial Infusion of Rapamycin in the Treatment of Rabbit Hepatocellular Carcinoma to Improve the Effect of TACE.Open life sciences · 2018Article
- Coexistence of Eph receptor B1 and ephrin B2 in port-wine stain endothelial progenitor cells contributes to clinicopathological vasculature dilatation.The British journal of dermatology · 2017Article
- Activation of PKCα and PI3K Kinases in Hypertrophic and Nodular Port Wine Stain Lesions.The American Journal of dermatopathology · 2017Article
- Intraoperative, real-time monitoring of blood flow dynamics associated with laser surgery of port wine stain birthmarks.Lasers in surgery and medicine · 2015Article
- Topical axitinib suppresses angiogenesis pathways induced by pulsed dye laser.The British journal of dermatology · 2015Article
- Topical rapamycin systematically suppresses the early stages of pulsed dye laser-induced angiogenesis pathways.Lasers in surgery and medicine · 2014Article
- Treatment of port wine stains with pulsed dye laser: a retrospective study of 848 cases in Shandong Province, People's Republic of China.Drug design, development and therapy · 2014Article
- Gentian violet: a 19th century drug re-emerges in the 21st century.Experimental dermatology · 2013Review
- Sturge-weber syndrome.Current treatment options in neurology · 2013Article
- Off-Label Topical Application of Sirolimus (Rapamycin) for Dermatological Conditions.Journal of cutaneous medicine and surgeryReview
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND AND
objectivesPulsed dye laser (PDL) is the most effective treatment for port wine stain (PWS) birthmarks. However, regeneration and revascularization of photocoagulated blood vessels may result in poor therapeutic outcome. We have recently shown that rapamycin (RPM), an angiogenesis inhibitor, can reduce the regeneration and revascularization of photocoagulated blood vessels. Herein, we attempt to further elucidate the molecular pathophysiology on the inhibition of the regeneration and revascularization of photocoagulated blood vessels by topical RPM in an animal model. MATERIALS AND
methodsTwo separate skin areas on each hamster were irradiated by PDL. After PDL exposure, topical RPM was applied daily to one of the randomly selected test sites. PDL, PDL + RPM and normal skin test sites were biopsied on day 3 after PDL exposure. The total ribonucleic acid (RNA) and protein were extracted from biopsied skin samples and quantified. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot were subsequently performed to quantify the mRNA and protein levels of hypoxia-inducible factor-1alpha (HIF-1α), vascular endothelial growth factor (VEGF) and ribosomal protein S6 kinase (S6). The phosphorylation levels of S6 and AKT were also evaluated by immunoblot.
resultsThe mRNA and protein levels of HIF-1α, VEGF, and S6 significantly increased after PDL exposure as compared to the normal hamster skin. Topical application of 1% RPM suppressed the PDL-induced increase in mRNA and protein levels of those genes on day 3 post-PDL exposure. The phosphorylation levels of S6 and AKT increased after PDL exposure but the increases were suppressed by the topical application of RPM.
conclusionThe increase in expression of HIF-1α, VEGF, and S6 after PDL-exposure suggests that angiogenesis pathways play very active roles in the process of skin blood vessel regeneration and revascularization. Topical application of 1% RPM can suppress the angiogenesis pathways and, therefore, reduce the regeneration and revascularization of photocoagulated blood vessels.
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