ArticleMolecular biology reports2013
Effects of cadmium exposure on sea urchin development assessed by SSH and RT-qPCR: metallothionein genes and their differential induction.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Between Life and Death: Sea Urchin Embryos Undergo Peculiar DNA Fragmentation after Exposure to Vanadium, Cadmium, Gadolinium, and Selenium.Life (Basel, Switzerland) · 2024Article
- Controlled uptake of PFOA in adult specimens of Paracentrotus lividus and evaluation of gene expression in their gonads and embryos.Environmental science and pollution research international · 2023Article
- Gene Expression Detects the Factors Influencing the Reproductive Success and the Survival Rates ofInternational journal of molecular sciences · 2022Article
- Toxicity of Vanadium during Development of Sea Urchin Embryos: Bioaccumulation, Calcium Depletion, ERK Modulation and Cell-Selective Apoptosis.International journal of molecular sciences · 2022Article
- PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea UrchinInternational journal of molecular sciences · 2021Article
- Two Benthic Diatoms,Marine drugs · 2021Article
- Article
- Combined Effects of Diatom-Derived Oxylipins on the Sea UrchinInternational journal of molecular sciences · 2020Article
- Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators.Cell stress & chaperones · 2019Review
- A Survey on Tubulin and Arginine Methyltransferase Families Sheds Light onInternational journal of molecular sciences · 2019Article
- HPV infection in semen: results from a new molecular approach.Epidemiology and infection · 2019Article
- Metallothionein expression and synthesis in the testis of the lizard Podarcis sicula under natural conditions and following estrogenic exposure.European journal of histochemistry : EJH · 2017Article
- Metallothionein Gene Family in the Sea Urchin Paracentrotus lividus: Gene Structure, Differential Expression and Phylogenetic Analysis.International journal of molecular sciences · 2017Article
- First Morphological and Molecular Evidence of the Negative Impact of Diatom-Derived Hydroxyacids on the Sea Urchin Paracentrotus lividus.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Identification of low Ca(2+) stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL).Plant biotechnology journal · 2016Article
- Differential sensitivity of snail embryos to cadmium: relation to age and metallothionein gene expression.Environmental science and pollution research international · 2016Article
- Autophagy as a defense strategy against stress: focus on Paracentrotus lividus sea urchin embryos exposed to cadmium.Cell stress & chaperones · 2016Review
- Maternal Exposure to Cadmium and Manganese Impairs Reproduction and Progeny Fitness in the Sea Urchin Paracentrotus lividus.PloS one · 2015Article
- Molecular response to toxic diatom-derived aldehydes in the sea urchin Paracentrotus lividus.Marine drugs · 2014Article
- The sea urchin metallothionein system: Comparative evaluation of the SpMTA and SpMTB metal-binding preferences.FEBS open bio · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to study the defense strategies activated by Paracentrotus lividus embryos in response to sub-lethal doses of CdCl2, we compared the induced transcripts to that of control embryos by suppression subtractive hybridization technique. We isolated five metallothionein (MT) cDNAs and other genes related to detoxification, to signaling pathway components, to oxidative, reductive and conjugative biotransformation, to RNA maturation and protein synthesis. RT-qPCR analysis revealed that two of the five P. lividus MT (PlMT7 and PlMT8) genes appeared to be constitutively expressed and upregulated following cadmium treatment, whereas the other three genes (PlMT4, PlMT5, PlMT6) are specifically switched-on in response to cadmium treatment. Moreover, we found that this transcriptional induction is concentration dependent and that the cadmium concentration threshold for the gene activation is distinct for every gene. RT-qPCR experiments showed in fact that, among induced genes, PlMT5 gene is activated at a very low cadmium concentration (0.1 μM) whereas PlMT4 and PlMT6 are activated at intermediate doses (1-10 μM). Differently, PlMT7 and PlMT8 genes increase significantly their expression only in embryos treated with the highest dose (100 μM CdCl2). We found also that, in response to a lethal dose of cadmium (1 μM), only PlMT5 and PlMT6 mRNA levels increased further. These data suggest a hierarchical and orchestrated response of the P. lividus embryo to overcome differential environmental stressors that could interfere with a normal development.
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