ArticleMolecular and cellular biochemistry2013
Treatment with hydrogen molecule alleviates TNFα-induced cell injury in osteoblast.
Article in Molecular and cellular biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Effects of Hydrogen-Rich Water Supplementation on Exercise Performance, Autonomic Nervous System Recovery, and Blood Lactate Concentration During Repeated Sprint Exercise in Male University Athletes.Journal of sports science & medicine · 2026Trial
- Molecular hydrogen and osteoarthritis: multi-target mechanisms and therapeutic effectiveness.Medical gas research · 2027Review
- Molecular Hydrogen and the Uremic Skeleton: A Critical Review and Turnover-State-Dependent Redox Hypothesis in CKD-MBD.Antioxidants (Basel, Switzerland) · 2026Review
- Mitigating mitochondrial dysfunction: a novel strategy for Chinese botanical drugs against osteoporosis.Frontiers in pharmacology · 2026Review
- Exploring the Potential of Molecular Hydrogen in Different Heart Failure Models: A Review.International journal of molecular sciences · 2025Review
- Molecular Hydrogen: From Molecular Effects to Stem Cells Management and Tissue Regeneration.Antioxidants (Basel, Switzerland) · 2023Review
- Molecular Hydrogen Prevents Osteoclast Activation in a Glucocorticoid-Induced Osteoporosis Zebrafish Scale Model.Antioxidants (Basel, Switzerland) · 2023Article
- Dynamics of Metabolic and Oxidative Parameters of Erythrocytes during Treatment of Chronic Heart Failure with Molecular Hydrogen.Bulletin of experimental biology and medicine · 2022Article
- Molecular hydrogen is a promising therapeutic agent for pulmonary disease.Journal of Zhejiang University. Science. B · 2022Article
- Hydrogen promotes the activation of Cu, Zn superoxide dismutase in a rat corneal alkali-burn model.International journal of ophthalmology · 2020Article
- Protective role of heme oxygenase-1 in fatty liver ischemia-reperfusion injury.Medical molecular morphology · 2019Review
- Recent Advances in Studies of Molecular Hydrogen against Sepsis.International journal of biological sciences · 2019Review
- Molecular hydrogen protects against ischemia-reperfusion injury in a mouse fatty liver model via regulating HO-1 and Sirt1 expression.Scientific reports · 2018Article
- CypD-mPTP axis regulates mitochondrial functions contributing to osteogenic dysfunction of MC3T3-E1 cells in inflammation.Journal of physiology and biochemistry · 2018Article
- Therapeutic Effect of Hydrogen Injected Subcutaneously on Onion Poisoned Dogs.Journal of veterinary research · 2017Article
- Hydrogen-rich saline prevents bone loss in diabetic rats induced by streptozotocin.International orthopaedics · 2017Article
- NADPH oxidases in bone homeostasis and osteoporosis.Cellular and molecular life sciences : CMLS · 2015Review
- Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles.Medical gas research · 2015Article
- Three hydrogen-rich solutions protect against intestinal injury in uncontrolled hemorrhagic shock.International journal of clinical and experimental medicine · 2015Article
- IL-10 counteracts proinflammatory mediator evoked oxidative stress in Caco-2 cells.Mediators of inflammation · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor necrosis factor-alpha (TNFα) plays a crucial role in inflammatory diseases such as rheumatoid arthritis and postmenopausal osteoporosis. Recently, it has been demonstrated that hydrogen gas, known as a novel antioxidant, can exert therapeutic anti-inflammatory effect in many diseases. In this study, we investigated the effect of treatment with hydrogen molecule (H(2)) on TNFα-induced cell injury in osteoblast. The osteoblasts isolated from neonatal rat calvariae were cultured. It was found that TNFα suppressed cell viability, induced cell apoptosis, suppressed Runx2 mRNA expression, and inhibited alkaline phosphatase activity, which was reversed by co-incubation with H(2). Incubation with TNFα-enhanced intracellular reactive oxygen species (ROS) formation and malondialdehyde production increased NADPH oxidase activity, impaired mitochondrial function marked by increased mitochondrial ROS formation and decreased mitochondrial membrane potential and ATP synthesis, and suppressed activities of antioxidant enzymes including SOD and catalase, which were restored by co-incubation with H(2). Treatment with H(2) inhibited TNFα-induced activation of NFκB pathway. In addition, treatment with H(2) inhibited TNFα-induced nitric oxide (NO) formation through inhibiting iNOS activity. Treatment with H(2) inhibited TNFα-induced IL-6 and ICAM-1 mRNA expression. In conclusion, treatment with H(2) alleviates TNFα-induced cell injury in osteoblast through abating oxidative stress, preserving mitochondrial function, suppressing inflammation, and enhancing NO bioavailability.
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