ArticleMolecular systems biology2012
Targeted proteomics reveals compositional dynamics of 60S pre-ribosomes after nuclear export.
Article in Molecular systems biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.Nature communications · 2025Article
- Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits.Nucleic acids research · 2025Article
- The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis.Nucleic acids research · 2024Article
- RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing.Nature communications · 2023Article
- Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.Nature structural & molecular biology · 2023Article
- Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.Nature structural & molecular biology · 2022Article
- Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events.Nucleic acids research · 2021Article
- Quantitative Mass Spectrometry-Based Proteomics for Biomarker Development in Ovarian Cancer.Molecules (Basel, Switzerland) · 2021Review
- The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes.Nucleic acids research · 2020Article
- From Snapshots to Flipbook-Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes.International journal of molecular sciences · 2020Review
- Article
- Shaping the Nascent Ribosome: AAA-ATPases in Eukaryotic Ribosome Biogenesis.Biomolecules · 2019Review
- The L1 stalk is required for efficient export of nascent large ribosomal subunits in yeast.RNA (New York, N.Y.) · 2019Article
- Conserved phosphorylation hotspots in eukaryotic protein domain families.Nature communications · 2019Article
- Ribosome assembly coming into focus.Nature reviews. Molecular cell biology · 2019Review
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Construction and intracellular targeting of eukaryotic pre-ribosomal particles involve a multitude of diverse transiently associating trans-acting assembly factors, energy-consuming enzymes, and transport factors. The ability to rapidly and reliably measure co-enrichment of multiple factors with maturing pre-ribosomal particles presents a major biochemical bottleneck towards revealing their function and the precise contribution of >50 energy-consuming steps that drive ribosome assembly. Here, we devised a workflow that combines genetic trapping, affinity-capture, and selected reaction monitoring mass spectrometry (SRM-MS), to overcome this deficiency. We exploited this approach to interrogate the dynamic proteome of pre-60S particles after nuclear export. We uncovered assembly factors that travel with pre-60S particles to the cytoplasm, where they are released before initiating translation. Notably, we identified a novel shuttling factor that facilitates nuclear export of pre-60S particles. Capturing and quantitating protein interaction networks of trapped intermediates of macromolecular complexes by our workflow is a reliable discovery tool to unveil dynamic processes that contribute to their in vivo assembly and transport.
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