ArticleInvestigative ophthalmology & visual science2013
The effect of size and species on lens intracellular hydrostatic pressure.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Extrinsic Modulation of the Zonular Tension Induces Calcium Influx in Lens Fiber Cells That Is Mediated by Mechanosensitive TRPV1 and TRPV4 Channels.Investigative ophthalmology & visual science · 2026Article
- Studies on Hydrostatic Pressure Responses to Piezo1 and TRPV4 in the Mouse Lens.Investigative ophthalmology & visual science · 2025Article
- Comparative analysis of rodent lens morphometrics and biomechanical properties.Frontiers in ophthalmology · 2025Article
- Regulation of water flow in the ocular lens: new roles for aquaporins.The Journal of physiology · 2024Review
- Modulation of Membrane Trafficking of AQP5 in the Lens in Response to Changes in Zonular Tension Is Mediated by the Mechanosensitive Channel TRPV1.International journal of molecular sciences · 2023Article
- Lens Aquaporins in Health and Disease: Location is Everything!Frontiers in physiology · 2022Review
- Proposed Role for Internal Lens Pressure as an Initiator of Age-Related Lens Protein Aggregation Diseases.Clinical ophthalmology (Auckland, N.Z.) · 2022Article
- Regulation of the Membrane Trafficking of the Mechanosensitive Ion Channels TRPV1 and TRPV4 by Zonular Tension, Osmotic Stress and Activators in the Mouse Lens.International journal of molecular sciences · 2021Article
- Connexin Gap Junctions and Hemichannels in Modulating Lens Redox Homeostasis and Oxidative Stress in Cataractogenesis.Antioxidants (Basel, Switzerland) · 2021Review
- Age-Dependent Changes in Total and Free Water Content of In Vivo Human Lenses Measured by Magnetic Resonance Imaging.Investigative ophthalmology & visual science · 2021Article
- Aquaporins Have Regional Functions in Development of Refractive Index in the Zebrafish Eye Lens.Investigative ophthalmology & visual science · 2021Article
- Ion Transport Regulation by TRPV4 and TRPV1 in Lens and Ciliary Epithelium.Frontiers in physiology · 2021Review
- Changes to Zonular Tension Alters the Subcellular Distribution of AQP5 in Regions of Influx and Efflux of Water in the Rat Lens.Investigative ophthalmology & visual science · 2020Article
- Signaling Between TRPV1/TRPV4 and Intracellular Hydrostatic Pressure in the Mouse Lens.Investigative ophthalmology & visual science · 2020Article
- TRPV1 activation stimulates NKCC1 and increases hydrostatic pressure in the mouse lens.American journal of physiology. Cell physiology · 2020Article
- The Ciliary Muscle and Zonules of Zinn Modulate Lens Intracellular Hydrostatic Pressure Through Transient Receptor Potential Vanilloid Channels.Investigative ophthalmology & visual science · 2019Article
- Verification and spatial mapping of TRPV1 and TRPV4 expression in the embryonic and adult mouse lens.Experimental eye research · 2019Article
- Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation.Investigative ophthalmology & visual science · 2019Article
- A Bidomain Model for Lens Microcirculation.Biophysical journal · 2019Article
- Disruption of the lens circulation causes calcium accumulation and precipitates in connexin mutant mice.American journal of physiology. Cell physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposePrevious experiments showed that mouse lenses have an intracellular hydrostatic pressure that varied from 335 mm Hg in central fibers to 0 mm Hg in surface cells. Model calculations predicted that in larger lenses, all else equal, pressure should increase as the lens radius squared. To test this prediction, lenses of different radii from different species were studied.
methodsAll studies were done in intact lenses. Intracellular hydrostatic pressures were measured with a microelectrode-manometer-based system. Membrane conductances were measured by frequency domain impedance analysis. Intracellular Na(+) concentrations were measured by injecting the Na(+)-sensitive dye sodium-binding benzofuran isophthalate.
resultsIntracellular hydrostatic pressures were measured in lenses from mice, rats, rabbits, and dogs with radii (cm) 0.11, 0.22, 0.49, and 0.57, respectively. In each species, pressure varied from 335 ± 6 mm Hg in central fiber cells to 0 mm Hg in surface cells. Further characterization of transport in lenses from mice and rats showed that the density of fiber cell gap junction channels was approximately the same, intracellular Na(+) concentrations varied from 17 mM in central fiber cells to 7 mM in surface cells, and intracellular voltages varied from -45 mV in central fiber cells to -60 mV in surface cells. Fiber cell membrane conductance was a factor of 2.7 times larger in mouse than in rat lenses.
conclusionsIntracellular hydrostatic pressure is an important physiological parameter that is regulated in lenses from these different species. The most likely mechanism of regulation is to reduce the density of open Na(+)-leak channels in fiber cells of larger lenses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.