ArticlePlacenta2013
Pericellular oxygen concentration of cultured primary human trophoblasts.
Article in Placenta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Strategies to capitalize on cell spheroid therapeutic potential for tissue repair and disease modeling.NPJ Regenerative medicine · 2022Review
- Two- and three-dimensional in vitro nucleus pulposus cultures: An in silico analysis of local nutrient microenvironments.JOR spine · 2022Article
- Modelling preeclampsia: a comparative analysis of the common human trophoblast cell lines.FASEB bioAdvances · 2021Article
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.Frontiers in endocrinology · 2020Review
- AP39, a Modulator of Mitochondrial Bioenergetics, Reduces Antiangiogenic Response and Oxidative Stress in Hypoxia-Exposed Trophoblasts: Relevance for Preeclampsia Pathogenesis.The American journal of pathology · 2019Article
- Limitations of oxygen delivery to cells in culture: An underappreciated problem in basic and translational research.Free radical biology & medicine · 2017Review
- Using stem cell oxygen physiology to optimize blastocyst culture while minimizing hypoxic stress.Journal of assisted reproduction and genetics · 2017Article
- Measurement of oxygen tension within mesenchymal stem cell spheroids.Journal of the Royal Society, Interface · 2017Article
- Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption.Journal of visualized experiments : JoVE · 2016Article
- Studies of the dynamics of nuclear clustering in human syncytiotrophoblast.Reproduction (Cambridge, England) · 2016Article
- Validation of murine and human placental explant cultures for use in sex steroid and phase II conjugation toxicology studies.Toxicology in vitro : an international journal published in association with BIBRA · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
introductionOxygen is pivotal in placental development and function. In vitro culture of human trophoblasts provides a useful model to study this phenomenon, but a hotly debated issue is whether or not the oxygen tension of the culture conditions mimics in vivo conditions. We tested the hypothesis that ambient oxygen tensions in culture reflect the pericellular oxygen levels.
methodsWe used a microelectrode oxygen sensor to measure the concentration of dissolved oxygen in the culture medium equilibrated with 21%, 8% or <0.5% oxygen.
resultsThe concentration of oxygen in medium without cells resembled that in the ambient atmosphere. The oxygen concentration present in medium bathing trophoblasts was remarkably dependent on the depth within the medium where sampling occurred, and the oxygen concentration within the overlying atmosphere was not reflected in medium immediately adjacent to the cells. Indeed, the pericellular oxygen concentration was in a range that most would consider severe hypoxia, at ≤0.6% oxygen or about 4.6 mm Hg, when the overlying atmosphere was 21% oxygen.
conclusionsWe conclude that culture conditions of 21% oxygen are unable to replicate the pO(2) of 40-60 mm Hg commonly attributed to the maternal blood in the intervillous space in the second and third trimesters of pregnancy. We further surmise that oxygen atmospheres in culture conditions between 0.5% and 21% provide different oxygen fluxes in the immediate pericellular environment yet can still yield insights into the responses of human trophoblast to different oxygen conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.