ArticlePloS one2012
Estimating microtubule distributions from 2D immunofluorescence microscopy images reveals differences among human cultured cell lines.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Generative machine learning unlocks the first proteome-wide image of human cells.bioRxiv : the preprint server for biology · 2026Article
- Microtubule regulation in cancer cells.Frontiers in cell and developmental biology · 2025Article
- Antibody Self-Assembly Maximizes Cytoplasmic Immunostaining Accuracy of Compact Quantum Dots.Chemistry of materials : a publication of the American Chemical Society · 2021Article
- CellOrganizer: Learning and Using Cell Geometries for Spatial Cell Simulations.Methods in molecular biology (Clifton, N.J.) · 2019Article
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- A microtubule-based minimal model for spontaneous and persistent spherical cell polarity.PloS one · 2017Article
- Building cell models and simulations from microscope images.Methods (San Diego, Calif.) · 2016Review
- Unbiased Rare Event Sampling in Spatial Stochastic Systems Biology Models Using a Weighted Ensemble of Trajectories.PLoS computational biology · 2016Article
- The Role of the Cytoskeleton and Myosin-Vc in the Targeting of KCa3.1 to the Basolateral Membrane of Polarized Epithelial Cells.Frontiers in physiology · 2016Article
- Automated Learning of Subcellular Variation among Punctate Protein Patterns and a Generative Model of Their Relation to Microtubules.PLoS computational biology · 2015Article
- Spatio-temporal Dynamics and Mechanisms of Stress Granule Assembly.PLoS computational biology · 2015Article
- In vivo imaging of Nematostella vectensis embryogenesis and late development using fluorescent probes.BMC cell biology · 2014Article
- Article
- Role of Phosphorylation and Hyperphosphorylation of Tau in Its Interaction with βα Dimeric Tubulin Studied from a Bioinformatics Perspective.Avicenna journal of medical biotechnologyArticle
- Extraction of Individual Filaments from 2D Confocal Microscopy Images of Flat Cells.IEEE/ACM transactions on computational biology and bioinformaticsArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Microtubules are filamentous structures that are involved in several important cellular processes, including cell division, cellular structure and mechanics, and intracellular transportation. Little is known about potential differences in microtubule distributions within and across cell lines. Here we describe a method to estimate information pertaining to 3D microtubule distributions from 2D fluorescence images. Our method allows for quantitative comparisons of microtubule distribution parameters (number of microtubules, mean length) between different cell lines. Among eleven cell lines compared, some showed differences that could be accounted for by differences in the total amount of tubulin per cell while others showed statistically significant differences in the balance between number and length of microtubules. We also observed that some cell lines that visually appear different in their microtubule distributions are quite similar when the model parameters are considered. The method is expected to be generally useful for comparing microtubule distributions between cell lines and for a given cell line after various perturbations. The results are also expected to enable analysis of the differences in gene expression underlying the observed differences in microtubule distributions among cell types.
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Registered trials
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