ArticlePloS one2012
Multiple-level regulation of 2,4-diacetylphloroglucinol production by the sigma regulator PsrA in Pseudomonas fluorescens 2P24.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Discovery and Comprehensive Characterization ofInternational journal of molecular sciences · 2026Article
- GacA uses two distinct regulatory mechanisms to control the biosynthesis of 2,4-diacetylphloroglucinol inApplied and environmental microbiology · 2025Article
- The flavonoid-sensing regulator AefR is involved in modulating quorum sensing through repressing the MexEF-OprN efflux pump inmSystems · 2025Article
- New Insights intoAntibiotics (Basel, Switzerland) · 2024Review
- Soil bacterium manipulates antifungal weapons by sensing intracellular type IVA secretion system effectors of a competitor.The ISME journal · 2023Article
- Article
- Genetic Circuit Design in Rhizobacteria.Biodesign research · 2022Review
- Transcriptomic analysis ofMicrobial genomics · 2022Article
- Exploring the expression and functionality of theRNA biology · 2021Article
- Pleiotropic effects of RsmA and RsmE proteins in Pseudomonas fluorescens 2P24.BMC microbiology · 2020Article
- PhlG mediates the conversion of DAPG to MAPG in Pseudomonas fluorescens 2P24.Scientific reports · 2020Article
- PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.Current microbiology · 2019Article
- An Extract Produced byFrontiers in microbiology · 2019Article
- Antagonistic Potential of Fluorescent Pseudomonads Colonizing Wheat Heads Against Mycotoxin Producing Alternaria and Fusaria.Frontiers in microbiology · 2018Article
- Posttranscriptional regulation of 2,4-diacetylphloroglucinol production by GidA and TrmE in Pseudomonas fluorescens 2P24.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas fluorescens 2P24 is a rhizospheric bacterium that aggressively colonizes the plant roots. It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The biosynthesis of 2,4-DAPG is regulated at the transcriptional level in the expression of the phlACBD operon as well as at the posttranscriptional level by the Gac/Rsm signal transduction pathway. However, the detailed mechanism of such regulation is not clear. METHODOLOGY/PRINCIPAL
findingsIn this study, we identified a binding site for the sigma regulator PsrA in the promoter region of the phlA gene. Electrophoretic mobility shift experiments revealed direct and specific binding of PsrA to the phlA promoter region. Consistent with the fact that its binding site locates within the promoter region of phlA, PsrA negatively regulates phlA expression, and its inactivation led to significant increase in 2,4-DAPG production. Interestingly, PsrA also activates the expression of the sigma factor RpoS, which negatively regulates 2,4-DAPG production by inducing the expression of the RNA-binding protein RsmA. CONCLUSIONS/SIGNIFICANCE: These results suggest that PsrA is an important regulator that modulates 2,4-DAPG biosynthesis at both transcriptional and posttranscriptional levels.
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