ArticlePloS one2012
Generating embryonic stem cells from the inbred mouse strain DBA/2J, a model of glaucoma and other complex diseases.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency.Cell stem cell · 2020Article
- Using the GEMM-ESC strategy to study gene function in mouse models.Nature protocols · 2015Article
- Combinatorial targeting of early pathways profoundly inhibits neurodegeneration in a mouse model of glaucoma.Neurobiology of disease · 2014Article
- Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains.Nature protocols · 2014Article
- Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.EMBO molecular medicine · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Mouse embryonic stem (ES) cells are derived from the inner cell mass of blastocyst stage embryos and are used primarily for the creation of genetically engineered strains through gene targeting. While some inbred strains of mice are permissive to the derivation of embryonic stem cell lines and are therefore easily engineered, others are nonpermissive or recalcitrant. Genetic engineering of recalcitrant strain backgrounds requires gene targeting in a permissive background followed by extensive backcrossing of the engineered allele into the desired strain background. The inbred mouse strain DBA/2J is a recalcitrant strain that is used as a model of many human diseases, including glaucoma, deafness and schizophrenia. Here, we describe the generation of germ-line competent ES cell lines derived from DBA/2J mice. We also demonstrate the utility of DBA/2J ES cells with the creation of conditional knockout allele for Endothelin-2 (Edn2) directly on the DBA/2J strain background.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.