ArticlePloS one2012
Sorting live stem cells based on Sox2 mRNA expression.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Downstream bioprocessing of human pluripotent stem cell-derived therapeutics.Engineering in life sciences · 2022Review
- FISH-TAMB, a Fixation-Free mRNA Fluorescent Labeling Technique to Target Transcriptionally Active Members in Microbial Communities.Microbial ecology · 2022Article
- Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.Biotechnology journal · 2022Article
- FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry.Nature protocols · 2017Article
- RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.BMC genomics · 2016Article
- Single-cell differences in matrix gene expression do not predict matrix deposition.Nature communications · 2016Article
- Flow Cytometric Characterization of Antigen-Specific T Cells Based on RNA and Its Advantages in Detecting Infections and Immunological Disorders.Critical reviews in immunology · 2016Article
- Molecular beacon-based detection and isolation of working-type cardiomyocytes derived from human pluripotent stem cells.Biomaterials · 2015Article
- Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential.Stem cell research & therapy · 2014Article
- Transcriptional profiling of cells sorted by RNA abundance.Nature methods · 2014Article
- The future of neuroepigenetics in the human brain.Progress in molecular biology and translational science · 2014Review
- High expression of hTERT and stemness genes in BORIS/CTCFL positive cells isolated from embryonic cancer cells.PloS one · 2014Article
- Tracking neuronal marker expression inside living differentiating cells using molecular beacons.Frontiers in cellular neuroscience · 2013Article
- Self-renewal of neural stem cells: implications for future therapies.Frontiers in physiology · 2013Article
- SOX2 and OCT4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary isolates. We show here how stem cells can be purified from mixed cell populations by sorting based on MBs. Specifically, we designed molecular beacons targeting Sox2, a well-known stem cell marker for murine embryonic (mES) and neural stem cells (NSC). One of our designed molecular beacons displayed an increase in fluorescence compared to a nonspecific molecular beacon both in vitro and in vivo when tested in mES and NSCs. We sorted Sox2-MB(+)SSEA1(+) cells from a mixed population of 4-day retinoic acid-treated mES cells and effectively isolated live undifferentiated stem cells. Additionally, Sox2-MB(+) cells isolated from primary mouse brains were sorted and generated neurospheres with higher efficiency than Sox2-MB(-) cells. These results demonstrate the utility of MBs for stem cell sorting in an mRNA-specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.