Trial reportThe Journal of biological chemistry2013
Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells.
Trial report in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 70 citations in OpenAlex.
- Facing Antimicrobial Resistance and Virulence in Streptococcus agalactiae During Late Pregnancy: Evaluation of Lactobacilli as a Supportive Approach.MicrobiologyOpen · 2026Article
- DeepVIC: modular prediction and classification of bacterial virulence factors using protein language model embeddings.Bioinformatics advances · 2026Article
- Cryo-EM structure of the conjugation H-pilus reveals the cyclic nature of the TrhA pilin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Infection of endothelial cells by Streptococcus agalactiae reveals potential role of PI-2b pilus on endothelial barrier dysfunction.Memorias do Instituto Oswaldo Cruz · 2025Article
- An opportunistic pathogen under stress: how Group B Streptococcus responds to cytotoxic reactive species and conditions of metal ion imbalance to survive.FEMS microbiology reviews · 2024Review
- Specific interaction between Group BInfection and immunity · 2024Article
- The Clinical and Genetic Characteristics ofInternational journal of molecular sciences · 2023Article
- Antibiotic resistance, biofilm formation, and virulence genes of Streptococcus agalactiae serotypes of Indian origin.BMC microbiology · 2023Article
- Relationship between Biofilm Production and High Somatic Cell Count inPathogens (Basel, Switzerland) · 2023Article
- Clonal Complex 12 Serotype Ib Streptococcus agalactiae Strain Causing Complicated Sepsis in Neonates: Clinical Features and Genetic Characteristics.Microbiology spectrum · 2023Article
- Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma.Life (Basel, Switzerland) · 2022Article
- Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing.Life (Basel, Switzerland) · 2022Article
- Streptococcal bacterial components in cancer therapy.Cancer gene therapy · 2022Review
- Bacterial and Host Determinants of Group B Streptococcal Infection of the Neonate and Infant.Frontiers in microbiology · 2022Review
- Genomic Characterization of Serotype III/ST-17 Group BBiomedicines · 2021Article
- Group B streptococcal infections in infants in Iceland: clinical and microbiological factors.Journal of medical microbiology · 2021Article
- Phenotype and multi-omics comparison of Staphylococcus and Streptococcus uncovers pathogenic traits and predicts zoonotic potential.BMC genomics · 2021Article
- Distribution of Pilus island and antibiotic resistance genes inIranian journal of microbiology · 2020Article
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus agalactiae, or group B Streptococcus (GBS), is an important opportunistic pathogen that causes pneumonia, sepsis, and meningitis in neonates and severe diseases in immunocompromised adults. We have performed comparative genomics of prevalent GBS serotypes of Indian origin (i.e. Ia, III, V, and VII). Pilus-proteins were commonly found up-regulated, and their expression was studied by using antiserum for GBS80 (backbone protein of pilus island-I), GBS67 (ancillary protein of PI-2a), and SAN1518 (backbone protein of PI-2b) by whole cell and Western blot analysis. To check the role of pilus proteins in adherence and invasion, an inhibition assay was performed. Comparative immunoblotting experiments revealed that expression of pili proteins does not differ in geographically different selected serotypes, Ia and V, of India and the United States. In the case of A549 cells, we found that GBS VII invasion and adherence was inhibited by pilus protein-specific antiserum SAN1518 significantly (p < 0.001) by 88.5 and 91%, respectively. We found that mutant strains, deficient in the pilus proteins (Δgbs80 and Δsan1518) exhibit a significant decrease in adherence in the case of type Ia, III, and VII. In the case of type VII, we have found a 95% reduction in invasion when Δsan1518 was used with A549 cells. Because the pilus proteins were identified previously as vaccine candidates against GBS serotypes of developed countries, we also found their role in the attachment and invasion of GBS of Indian origin. Thus, the present work supports the idea of making a more effective pilus protein-based vaccine that can be used universally.
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