ArticleMolecular and cellular biology2013
Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- uORF-mediated translational control of CAK and Cdk activation in response to low nutrients in yeast.EMBO reports · 2026Article
- Translational control of CAK and Cdk T-loop phosphorylation in response to growth in yeast.bioRxiv : the preprint server for biology · 2026Article
- Cdk activation by phosphorylation: linking growth signals to cell cycle control.Biochemical Society transactions · 2025Review
- Membrane and organelle rearrangement during ascospore formation in budding yeast.Microbiology and molecular biology reviews : MMBR · 2024Review
- The MAPK homolog, Smk1, promotes assembly of the glucan layer of the spore wall in S. cerevisiae.Yeast (Chichester, England) · 2024Article
- Meiotic Cytokinesis inJournal of fungi (Basel, Switzerland) · 2024Review
- Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CThe EMBO journal · 2023Article
- A Noncanonical Hippo Pathway Regulates Spindle Disassembly and Cytokinesis During Meiosis inGenetics · 2020Article
- Isc10, an Inhibitor That Links the Anaphase-Promoting Complex to a Meiosis-Specific Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2020Article
- Novel insights into gene expression regulation during meiosis revealed by translation elongation dynamics.NPJ systems biology and applications · 2019Article
- RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.Biochemistry · 2018Article
- The meiosis-specific Cdc20 family-member Ama1 promotes binding of the Ssp2 activator to the Smk1 MAP kinase.Molecular biology of the cell · 2018Article
- Ssp2 Binding Activates the Smk1 Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2017Article
- Cell Signaling and Stress Responses.Cold Spring Harbor perspectives in biology · 2016Review
- Timely Closure of the Prospore Membrane Requires SPS1 and SPO77 in Saccharomyces cerevisiae.Genetics · 2016Article
- Post-transcriptional regulation in budding yeast meiosis.Current genetics · 2016Review
- Autophosphorylation of the Smk1 MAPK is spatially and temporally regulated by Ssp2 during meiotic development in yeast.Molecular biology of the cell · 2015Article
- Multisite phosphorylation of the Sum1 transcriptional repressor by S-phase kinases controls exit from meiotic prophase in yeast.Molecular and cellular biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Smk1 is a meiosis-specific mitogen-activated protein kinase (MAPK) in Saccharomyces cerevisiae that controls spore morphogenesis. Similar to other MAPKs, it is controlled by dual phosphorylation of its T-X-Y activation motif. However, Smk1 is not phosphorylated by a prototypical MAPK kinase. Here, we show that the T residue in Smk1's activation motif is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1. The Y residue is autophosphorylated in an independent intramolecular reaction that requires the meiosis-specific protein Ssp2. Although both SMK1 and SSP2 are expressed as middle-meiosis-specific genes, Smk1 protein starts to accumulate before Ssp2. Thus, Smk1 exists in a low-activity (pT) form early in sporulation and a high-activity (pT/pY) form later in the program. Ssp2 must be present when Smk1 is being produced to activate the autophosphorylation reaction, suggesting that Ssp2 acts through a transitional intermediate form of Smk1. These findings provide a mechanistic explanation for how Smk1 activity thresholds are generated. They demonstrate that intramolecular autophosphorylation of MAPKs can be regulated and suggest new mechanisms for coupling MAPK outputs to developmental programs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.