ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2013
Using spin-label W-band EPR to study membrane fluidity profiles in samples of small volume.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
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- Curcumin-Lipid Interactions in PEGylated vs. Conventional Liposomes: A Combined Fluorescence and EPR Study.Membranes · 2026Article
- Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes.Membranes · 2022Article
- Molecular oxygen as a probe molecule in EPR spin-labeling studies of membrane structure and dynamics.Oxygen (Basel, Switzerland) · 2022Article
- Multilamellar Liposomes as a Model for Biological Membranes: Saturation Recovery EPR Spin-Labeling Studies.Membranes · 2022Review
- Factors determining barrier properties to oxygen transport across model and cell plasma membranes based on EPR spin-label oximetry.Applied magnetic resonance · 2021Article
- Differences in the properties of porcine cortical and nuclear fiber cell plasma membranes revealed by saturation recovery EPR spin labeling measurements.Experimental eye research · 2021Article
- Interaction of Alpha-Crystallin with Phospholipid Membranes.Current eye research · 2021Article
- Oxygen Transport Parameter in Plasma Membrane of Eye Lens Fiber Cells by Saturation Recovery EPR.Applied magnetic resonance · 2021Article
- Effects of Curcumin on Lipid Membranes: an EPR Spin-label Study.Cell biochemistry and biophysics · 2020Article
- Characterization of the distribution of spin-lattice relaxation rates of lipid spin labels in fiber cell plasma membranes of eye lenses with a stretched-exponential function.Applied magnetic resonance · 2019Article
- Autobiography of James S. Hyde.Applied magnetic resonance · 2017Article
- EPR Studies on the Properties of Model Photoreceptor Membranes Made of Natural and Synthetic Lipids.Cell biochemistry and biophysics · 2017Article
- High Cholesterol/Low Cholesterol: Effects in Biological Membranes: A Review.Cell biochemistry and biophysics · 2017Review
- Broadband W-band Rapid Frequency Sweep Considerations for Fourier Transform EPR.Cell biochemistry and biophysics · 2017Article
- Cholesterol Bilayer Domains in the Eye Lens Health: A Review.Cell biochemistry and biophysics · 2017Review
- Saturation recovery EPR spin-labeling method for quantification of lipids in biological membrane domains.Applied magnetic resonance · 2017Article
- Spin-labeled small unilamellar vesicles with theApplied magnetic resonance · 2015Article
- Lipid domains in intact fiber-cell plasma membranes isolated from cortical and nuclear regions of human eye lenses of donors from different age groups.Experimental eye research · 2015Article
- Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61-70-year-old human donors.European biophysics journal : EBJ · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Conventional and saturation-recovery (SR) EPR at W-band (94GHz) using phosphatidylcholine spin labels (labeled at the alkyl chain [n-PC] and headgroup [T-PC]) to obtain profiles of membrane fluidity has been demonstrated. Dimyristoylphosphatidylcholine (DMPC) membranes with and without 50 mol% cholesterol have been studied, and the results have been compared with similar studies at X-band (9.4 GHz) (L. Mainali, J.B. Feix, J.S. Hyde, W.K. Subczynski, J. Magn. Reson. 212 (2011) 418-425). Profiles of the spin-lattice relaxation rate (T(1)(-1)) obtained from SR EPR measurements for n-PCs and T-PC were used as a convenient quantitative measure of membrane fluidity. Additionally, spectral analysis using Freed's MOMD (microscopic-order macroscopic-disorder) model (E. Meirovitch, J.H. Freed J. Phys. Chem. 88 (1984) 4995-5004) provided rotational diffusion coefficients (R(perpendicular) and R(||)) and order parameters (S(0)). Spectral analysis at X-band provided one rotational diffusion coefficient, R(perpendicular). T(1)(-1), R(perpendicular), and R(||) profiles reflect local membrane dynamics of the lipid alkyl chain, while the order parameter shows only the amplitude of the wobbling motion of the lipid alkyl chain. Using these dynamic parameters, namely T(1)(-1), R(perpendicular), and R(||), one can discriminate the different effects of cholesterol at different depths, showing that cholesterol has a rigidifying effect on alkyl chains to the depth occupied by the rigid steroid ring structure and a fluidizing effect at deeper locations. The nondynamic parameter, S(0), shows that cholesterol has an ordering effect on alkyl chains at all depths. Conventional and SR EPR measurements with T-PC indicate that cholesterol has a fluidizing effect on phospholipid headgroups. EPR at W-band provides more detailed information about the depth-dependent dynamic organization of the membrane compared with information obtained at X-band. EPR at W-band has the potential to be a powerful tool for studying membrane fluidity in samples of small volume, ~30 nL, compared with a representative sample volume of ~3 μL at X-band.
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