ArticleAnalytical chemistry2012
Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap.Nature protocols · 2020Article
- Deep Profiling of Cellular Heterogeneity by Emerging Single-Cell Proteomic Technologies.Proteomics · 2020Review
- How single-cell immunology is benefiting from microfluidic technologies.Microsystems & nanoengineering · 2020Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Dynamic secretome of bone marrow-derived stromal cells reveals a cardioprotective biochemical cocktail.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies.European journal of immunology · 2017Article
- Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses.ACS nano · 2017Article
- Microchip-based single-cell functional proteomics for biomedical applications.Lab on a chip · 2017Review
- Bridging the gap: microfluidic devices for short and long distance cell-cell communication.Lab on a chip · 2017Review
- Single-Cell, Multiplexed Protein Detection of Rare Tumor Cells Based on a Beads-on-Barcode Antibody Microarray.Analytical chemistry · 2016Article
- Magnetophoretic transistors in a tri-axial magnetic field.Lab on a chip · 2016Article
- Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.Sensors (Basel, Switzerland) · 2016Review
- Article
- Stochastic particle barcoding for single-cell tracking and multiparametric analysis.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- High-throughput 3D cell invasion chip enables accurate cancer metastatic assays.Journal of the American Chemical Society · 2014Article
- Advances in high-throughput single-cell microtechnologies.Current opinion in biotechnology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
We present a method that uses fluorescent cellular barcodes to increase the number of unique samples that can be analyzed simultaneously by microengraving, a nanowell array-based technique for quantifying the secretory responses of thousands of single cells in parallel. Using n different fluorescent dyes to generate 2(n) unique cellular barcodes, we achieved a 2(n)-fold reduction in the number of arrays and quantity of reagents required per sample. The utility of this approach was demonstrated in three applications of interest in clinical and experimental immunology. Using barcoded human peripheral blood mononuclear cells and T cells, we constructed dose-response curves, profiled the secretory behavior of cells treated with mechanistically distinct stimuli, and tracked the secretory behaviors of different lineages of CD4(+) T helper cells. In addition to increasing the number of samples analyzed by generating secretory profiles of single cells from multiple populations in a time- and reagent-efficient manner, we expect that cellular barcoding in combination with microengraving will facilitate unique experimental opportunities for quantitatively analyzing interactions among heterogeneous cells isolated in small groups (~2-5 cells).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.