ArticleProceedings of the National Academy of Sciences of the United States of America1990
Isolation of a 5-kilodalton actin-sequestering peptide from human blood platelets.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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Who cites it
47 citing papers in PubMed, 172 citations in OpenAlex.
- Small molecule intervention of actin-binding protein profilin1 reduces tumor angiogenesis in renal cell carcinoma.bioRxiv : the preprint server for biology · 2025Article
- The role ofJournal of gastrointestinal oncology · 2025Article
- Pan-cancer analysis identifies the correlations of Thymosin Beta 10 with predicting prognosis and immunotherapy response.Frontiers in immunology · 2023Article
- Biochemical and mechanical regulation of actin dynamics.Nature reviews. Molecular cell biology · 2022Review
- TMSB10 promotes migration and invasion of cancer cells and is a novel prognostic marker for renal cell carcinoma.International journal of clinical and experimental pathology · 2019Article
- Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours.Rapid communications in mass spectrometry : RCM · 2018Article
- Thymosin beta 10 is a key regulator of tumorigenesis and metastasis and a novel serum marker in breast cancer.Breast cancer research : BCR · 2017Article
- A peptide from human β thymosin as a platform for the development of new anti-biofilm agents for Staphylococcus spp. and Pseudomonas aeruginosa.World journal of microbiology & biotechnology · 2016Article
- Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II.Molecular cytogenetics · 2016Article
- Structural basis of thymosin-β4/profilin exchange leading to actin filament polymerization.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Thymosin β4 and tissue transglutaminase. Molecular characterization of cyclic thymosin β4.The protein journal · 2013Article
- Expression patterns of Thymosin β4 and cancer stem cell marker CD133 in ovarian cancers.Pathology oncology research : POR · 2013Article
- Article
- Actin nucleators in the nucleus: an emerging theme.Journal of cell science · 2012Review
- Article
- Expression pattern of thymosin beta 4 in the adult human liver.European journal of histochemistry : EJH · 2011Article
- Immunoreactivity of thymosin beta 4 in human foetal and adult genitourinary tract.European journal of histochemistry : EJH · 2010Article
- Different thymosin Beta 4 immunoreactivity in foetal and adult gastrointestinal tract.PloS one · 2010Article
- Evaluation of skeletal and cardiac muscle function after chronic administration of thymosin beta-4 in the dystrophin deficient mouse.PloS one · 2010Article
- Characterization of the chondrocyte actin cytoskeleton in living three-dimensional culture: response to anabolic and catabolic stimuli.Molecular & cellular biomechanics : MCB · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Resting human platelets contain approximately 0.3 mM unpolymerized actin. When freshly drawn and washed platelets are treated with saponin, 85-90% of the unpolymerized actin diffuses out. Analysis by polyacrylamide gel electrophoresis under nondenaturing conditions shows that the bulk of this unpolymerized actin migrates with a higher mobility than does pure G-actin, profilactin, or actin-gelsolin complex. When muscle G-actin is added to fresh or boiled saponin extract, the added muscle actin is shifted to the high-mobility form. The saponin extract contains an acidic peptide having a molecular mass in the range of 5 kDa, which has been purified to homogeneity by reverse-phase HPLC. This peptide also shifts muscle actin to the high-mobility form. Addition of either boiled saponin extract or the purified peptide to muscle G-actin also strongly and stoichiometrically inhibits salt-induced polymerization, as assayed by falling-ball viscometry and by sedimentation. We conclude that this peptide binds to the bulk of the unpolymerized actin in platelets and prevents it from polymerizing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.