ArticleThe Journal of biological chemistry2013
Arabidopsis calmodulin-binding protein IQ67-domain 1 localizes to microtubules and interacts with kinesin light chain-related protein-1.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 123 citations in OpenAlex.
- Article
- A CaPlants (Basel, Switzerland) · 2026Article
- E69K mutation in β-tubulin 2 blocks cell wall integrity signaling during plant cell elongation.EMBO reports · 2025Article
- TaCML49-B, a Calmodulin-like Protein, Interacts with TaIQD23 to Positively Regulate Salt Tolerance in Wheat.Plants (Basel, Switzerland) · 2025Article
- GmIQD63 functions as a novel GmCDPK38-interacting protein in soybean defense against the common cutworm.Planta · 2025Article
- The IQ67-domain protein IQD1 regulates fruit shape through complex multiprotein interactions in pepper (Capsicum annuum L.).Plant biotechnology journal · 2025Article
- Review
- The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Developmental Morphology, Physiology, and Molecular Basis of the Pentagram Fruit ofPlants (Basel, Switzerland) · 2024Article
- Genome-Wide Characterization of IQD Family Proteins in Apple and Functional Analysis of the Microtubule-Regulating Abilities of MdIQD17 and MdIQD28 under Cold Stress.Plants (Basel, Switzerland) · 2024Article
- Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins.Journal of experimental botany · 2024Article
- Functional Analysis of thePlants (Basel, Switzerland) · 2024Article
- Morphological, histological and transcriptomic mechanisms underlying different fruit shapes inPeerJ · 2024Article
- Microtubule-associated protein SlMAP70 interacts with IQ67-domain protein SlIQD21a to regulate fruit shape in tomato.The Plant cell · 2023Article
- Molecular and genetic regulations of fleshy fruit shape and lessons fromHorticulture research · 2023Article
- The cotton protein GhIQD21 interacts with GhCaM7 and modulates organ morphogenesis in Arabidopsis by influencing microtubule stability.Plant cell reports · 2023Article
- Mutation ofInternational journal of molecular sciences · 2023Article
- Form and contour: breeding and genetics of organ shape from wild relatives to modern vegetable crops.Frontiers in plant science · 2023Review
- Integrative analysis of grapevine (Frontiers in nutrition · 2023Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2022Article
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9 authors at 2 institutions in 2 countries.
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Abstract
Calcium (Ca(2+)) is a key second messenger in eukaryotes and regulates diverse cellular processes, most notably via calmodulin (CaM). In Arabidopsis thaliana, IQD1 (IQ67 domain 1) is the founding member of the IQD family of putative CaM targets. The 33 predicted IQD proteins share a conserved domain of 67 amino acids that is characterized by a unique arrangement of multiple CaM recruitment motifs, including so-called IQ motifs. Whereas IQD1 has been implicated in the regulation of defense metabolism, the biochemical functions of IQD proteins remain to be elucidated. In this study we show that IQD1 binds to multiple Arabidopsis CaM and CaM-like (CML) proteins in vitro and in yeast two-hybrid interaction assays. CaM overlay assays revealed moderate affinity of IQD1 to CaM2 (K(d) ∼ 0.6 μm). Deletion mapping of IQD1 demonstrated the importance of the IQ67 domain for CaM2 binding in vitro, which is corroborated by interaction of the shortest IQD member, IQD20, with Arabidopsis CaM/CMLs in yeast. A genetic screen of a cDNA library identified Arabidopsis kinesin light chain-related protein-1 (KLCR1) as an IQD1 interactor. The subcellular localization of GFP-tagged IQD1 proteins to microtubules and the cell nucleus in transiently and stably transformed plant tissues (tobacco leaves and Arabidopsis seedlings) suggests direct interaction of IQD1 and KLCR1 in planta that is supported by GFP∼IQD1-dependent recruitment of RFP∼KLCR1 and RFP∼CaM2 to microtubules. Collectively, the prospect arises that IQD1 and related proteins provide Ca(2+)/CaM-regulated scaffolds for facilitating cellular transport of specific cargo along microtubular tracks via kinesin motor proteins.
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