ArticleThe Journal of biological chemistry2013
The bacterial actin MamK: in vitro assembly behavior and filament architecture.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Biosynthesis and function of magnetic organelles in magnetotactic bacteria.Nature reviews. Microbiology · 2026Review
- Visualizing the dynamic polymerization of the bacterial actin-like cytoskeleton for magnetic organelle positioning.Scientific reports · 2025Article
- Magnetosome organelles are organized through interactions between McaA and McaB that alter the dynamics of the bacterial actin-like protein MamK.bioRxiv : the preprint server for biology · 2025Article
- Organizing organelles: bacterial strategies for localizing intracellular compartments.Current opinion in microbiology · 2025Review
- A family of bacterial actin homologs forms a three-stranded tubular structure.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Archaeal actins and the origin of a multi-functional cytoskeleton.Journal of bacteriology · 2024Review
- Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover.Nature communications · 2024Article
- A Review of the Current State of Magnetic Force Microscopy to Unravel the Magnetic Properties of Nanomaterials Applied in Biological Systems and Future Directions for Quantum Technologies.Nanomaterials (Basel, Switzerland) · 2023Review
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- Article
- Current view of iron biomineralization in magnetotactic bacteria.Journal of structural biology: X · 2021Review
- A Compass To Boost Navigation: Cell Biology of Bacterial Magnetotaxis.Journal of bacteriology · 2020Review
- Metabolic characterisation ofRSC advances · 2020Article
- Magnetic genes: Studying the genetics of biomineralization in magnetotactic bacteria.PLoS genetics · 2020Review
- Cryo-EM reconstruction of AlfA fromProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Evolution of polymer formation within the actin superfamily.Molecular biology of the cell · 2017Review
- Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
It is now recognized that actin-like proteins are widespread in bacteria and, in contrast to eukaryotic actins, are highly diverse in sequence and function. The bacterial actin, MamK, represents a clade, primarily found in magnetotactic bacteria, that is involved in the proper organization of subcellular organelles, termed magnetosomes. We have previously shown that MamK from Magnetospirillum magneticum AMB-1 (AMB-1) forms dynamic filaments in vivo. To gain further insights into the molecular mechanisms that underlie MamK dynamics and function, we have now studied the in vitro properties of MamK. We demonstrate that MamK is an ATPase that, in the presence of ATP, assembles rapidly into filaments that disassemble once ATP is depleted. The mutation of a conserved active site residue (E143A) abolishes ATPase activity of MamK but not its ability to form filaments. Filament disassembly depends on both ATPase activity and potassium levels, the latter of which results in the organization of MamK filaments into bundles. These data are consistent with observations indicating that accessory factors are required to promote filament disassembly and for spatial organization of filaments in vivo. We also used cryo-electron microscopy to obtain a high resolution structure of MamK filaments. MamK adopts a two-stranded helical filament architecture, but unlike eukaryotic actin and other actin-like filaments, subunits in MamK strands are unstaggered giving rise to a unique filament architecture. Beyond extending our knowledge of the properties and function of MamK in magnetotactic bacteria, this study emphasizes the functional and structural diversity of bacterial actins in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.