ArticleJournal of bacteriology2013
Role of the CipA scaffoldin protein in cellulose solubilization, as determined by targeted gene deletion and complementation in Clostridium thermocellum.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Improving cellulosic ethanol production by an engineered yeast consortium displaying a pentafunctional mini-cellulosome.FEMS yeast research · 2025Article
- Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings.Biotechnology for biofuels · 2021Article
- CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313.Microbial biotechnology · 2020Article
- Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum.Microbial biotechnology · 2018Article
- Regulation of biomass degradation by alternative σ factors in cellulolytic clostridia.Scientific reports · 2018Article
- Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum.Applied and environmental microbiology · 2017Article
- How does cellulosome composition influence deconstruction of lignocellulosic substrates inBiotechnology for biofuels · 2017Article
- Efficient whole-cell-catalyzing cellulose saccharification using engineeredBiotechnology for biofuels · 2017Article
- Identification and Characterization of a Large Protein Essential for Degradation of the Crystalline Region of Cellulose by Cytophaga hutchinsonii.Applied and environmental microbiology · 2017Article
- Development of a plasmid-based expression system inMetabolic engineering communications · 2016Article
- Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass.Scientific reports · 2016Article
- Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities.Science advances · 2016Article
- Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum.Biotechnology for biofuels · 2016Article
- Identifying promoters for gene expression inMetabolic engineering communications · 2015Article
- Stoichiometric Assembly of the Cellulosome Generates Maximum Synergy for the Degradation of Crystalline Cellulose, as Revealed by In Vitro Reconstitution of the Clostridium thermocellum Cellulosome.Applied and environmental microbiology · 2015Article
- A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum.Biotechnology for biofuels · 2015Article
- The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading.Biotechnology for biofuels · 2014Article
- Inference of phenotype-defining functional modules of protein families for microbial plant biomass degraders.Biotechnology for biofuels · 2014Article
- The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons.Biotechnology for biofuels · 2014Article
- Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CipA scaffoldin protein plays a key role in the Clostridium thermocellum cellulosome. Previous studies have revealed that mutants deficient in binding or solubilizing cellulose also exhibit reduced expression of CipA. To confirm that CipA is, in fact, necessary for rapid solubilization of crystalline cellulose, the gene was deleted from the chromosome using targeted gene deletion technologies. The CipA deletion mutant exhibited a 100-fold reduction in cellulose solubilization rate, although it was eventually able to solubilize 80% of the 5 g/liter cellulose initially present. The deletion mutant was complemented by a copy of cipA expressed from a replicating plasmid. In this strain, Avicelase activity was restored, although the rate was 2-fold lower than that in the wild type and the duration of the lag phase was increased. The cipA coding sequence is located at the beginning of a gene cluster containing several other genes thought to be responsible for the structural organization of the cellulosome, including olpB, orf2p, and olpA. Tandem mass spectrometry revealed a 10-fold reduction in the expression of olpB, which may explain the lower growth rate. This deletion experiment adds further evidence that CipA plays a key role in cellulose solubilization by C. thermocellum, and it raises interesting questions about the differential roles of the anchor scaffoldin proteins OlpB, Orf2p, and SdbA.
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