ArticleApplied and environmental microbiology2013
A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 202 citations in OpenAlex.
- Overexpression of endogenous aldehyde:ferredoxin oxidoreductase genes enhances ethanol biosynthesis in Clostridium autoethanogenum.World journal of microbiology & biotechnology · 2026Article
- Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi.Applied microbiology and biotechnology · 2026Article
- Optimization of Plasmid Curing from Genetically EngineeredACS synthetic biology · 2025Article
- A thermostable Cas9-based genome editing system for thermophilic acetogenic bacteriumApplied and environmental microbiology · 2025Article
- Genetic tools for the electrotrophApplied and environmental microbiology · 2024Article
- Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP.Microbial cell factories · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- Heterologous Production of Isopropanol Using Metabolically EngineeredBioengineering (Basel, Switzerland) · 2023Article
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.Biotechnology for biofuels and bioproducts · 2023Article
- Deletion of genes linked to the CFrontiers in bioengineering and biotechnology · 2023Article
- Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum.Journal of industrial microbiology & biotechnology · 2022Article
- Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from COMicrobial cell factories · 2022Article
- Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences.Nucleic acids research · 2022Article
- EstablishingApplied and environmental microbiology · 2022Article
- Microbial COArchives of microbiology · 2022Review
- Acetogenic production of 3-Hydroxybutyrate using a native 3-Hydroxybutyryl-CoA Dehydrogenase.Frontiers in microbiology · 2022Article
- Biosynthesis Based on One-Carbon Mixotrophy.Advances in biochemical engineering/biotechnology · 2022Article
- Hexanol biosynthesis from syngas byHeliyon · 2021Article
- Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum.Biotechnology for biofuels · 2021Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methods for genetic manipulation of Clostridium ljungdahlii are of interest because of the potential for production of fuels and other biocommodities from carbon dioxide via microbial electrosynthesis or more traditional modes of autotrophy with hydrogen or carbon monoxide as the electron donor. Furthermore, acetogenesis plays an important role in the global carbon cycle. Gene deletion strategies required for physiological studies of C. ljungdahlii have not previously been demonstrated. An electroporation procedure for introducing plasmids was optimized, and four different replicative origins for plasmid propagation in C. ljungdahlii were identified. Chromosomal gene deletion via double-crossover homologous recombination with a suicide vector was demonstrated initially with deletion of the gene for FliA, a putative sigma factor involved in flagellar biogenesis and motility in C. ljungdahlii. Deletion of fliA yielded a strain that lacked flagella and was not motile. To evaluate the potential utility of gene deletions for functional genomic studies and to redirect carbon and electron flow, the genes for the putative bifunctional aldehyde/alcohol dehydrogenases, adhE1 and adhE2, were deleted individually or together. Deletion of adhE1, but not adhE2, diminished ethanol production with a corresponding carbon recovery in acetate. The double deletion mutant had a phenotype similar to that of the adhE1-deficient strain. Expression of adhE1 in trans partially restored the capacity for ethanol production. These results demonstrate the feasibility of genetic investigations of acetogen physiology and the potential for genetic manipulation of C. ljungdahlii to optimize autotrophic biocommodity production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.