ArticleEukaryotic cell2013
Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Echinocandin tolerance and persistencemBio · 2026Article
- Calcium Transport and Enrichment in Microorganisms: A Review.Foods (Basel, Switzerland) · 2024Review
- Protein Kinase C Is Involved in Vegetative Development, Stress Response and Pathogenicity inInternational journal of molecular sciences · 2023Article
- Review
- Modeling Calcium Signaling inFrontiers in molecular biosciences · 2022Article
- Overview ofIndian journal of microbiology · 2020Review
- Vacuolar zinc transporter Zrc1 is required for detoxification of excess intracellular zinc in the human fungal pathogen Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2018Article
- HG3 (Bethesda, Md.) · 2017Article
- Reciprocal Regulation of Target of Rapamycin Complex 1 and Potassium Accumulation.The Journal of biological chemistry · 2017Article
- Identification of Genes in Candida glabrata Conferring Altered Responses to Caspofungin, a Cell Wall Synthesis Inhibitor.G3 (Bethesda, Md.) · 2016Article
- Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration.Biology open · 2016Article
- A LAPF/phafin1-like protein regulates TORC1 and lysosomal membrane permeabilization in response to endoplasmic reticulum membrane stress.Molecular biology of the cell · 2015Article
- Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains.The Journal of biological chemistry · 2015Article
- Complementation of the Yeast Model System Reveals that Caenorhabditis elegans OCT-1 Is a Functional Transporter of Anthracyclines.PloS one · 2015Article
- Kch1 family proteins mediate essential responses to endoplasmic reticulum stresses in the yeasts Saccharomyces cerevisiae and Candida albicans.The Journal of biological chemistry · 2013Article
- Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In the budding yeast Saccharomyces cerevisiae, mating pheromones activate a high-affinity Ca(2+) influx system (HACS) that activates calcineurin and is essential for cell survival. Here we identify extracellular K(+) and a homologous pair of transmembrane proteins, Kch1 and Kch2 (Prm6), as necessary components of the HACS activation mechanism. Expression of Kch1 and especially Kch2 was strongly induced during the response to mating pheromones. When forcibly overexpressed, Kch1 and Kch2 localized to the plasma membrane and activated HACS in a fashion that depended on extracellular K(+) but not pheromones. They also promoted growth of trk1 trk2 mutant cells in low K(+) environments, suggesting they promote K(+) uptake. Voltage-clamp recordings of protoplasts revealed diminished inward K(+) currents in kch1 kch2 double-mutant cells relative to the wild type. Conversely, heterologous expression of Kch1 in HEK293T cells caused the appearance of inwardly rectifying K(+) currents. Collectively, these findings suggest that Kch1 and Kch2 directly promote K(+) influx and that HACS may electrochemically respond to K(+) influx in much the same way as the homologous voltage-gated Ca(2+) channels in most animal cell types.
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