ArticleArchives of toxicology2013
A 3-dimensional human embryonic stem cell (hESC)-derived model to detect developmental neurotoxicity of nanoparticles.
Article in Archives of toxicology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 131 citations in OpenAlex.
- A genomics-based framework for identifying biomarkers of human neurodevelopmental toxicity.Reproductive toxicology (Elmsford, N.Y.) · 2016Pooled it
- Human Stem Cell-Derived Neurospheroids and a Multi-Assay Strategy Provide Brain Cytotoxicity New Insights from ADB-FUBIATA Exposure.Neurotoxicity research · 2026Article
- Exposure to Nanoplastics Disrupts Neurotransmitter Release in Rat Hippocampal Neurons.Environment & health (Washington, D.C.) · 2026Article
- Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.International journal of molecular sciences · 2025Review
- The Presence of Micro- and Nanoplastics in Food and the Estimation of the Amount Consumed Depending on Dietary Patterns.Molecules (Basel, Switzerland) · 2025Review
- Differential lncRNA Expression in Undifferentiated and Differentiated LUHMES Cells Following Co-Exposure to Silver Nanoparticles and Nanoplastic.Materials (Basel, Switzerland) · 2025Article
- Neurotoxicity and Developmental Neurotoxicity of Copper Sulfide Nanoparticles on a Human Neuronal In-Vitro Test System.International journal of molecular sciences · 2024Article
- PLASTAMINATION: Outcomes on the Central Nervous System and Reproduction.Current neuropharmacology · 2024Review
- Anionic nanoplastic contaminants promote Parkinson's disease-associated α-synuclein aggregation.Science advances · 2023Article
- Personal protective equipment and micro-nano plastics: A review of an unavoidable interrelation for a global well-being hazard.Hygiene and environmental health advances · 2023Review
- Human Astrocyte Spheroids as Suitable In Vitro Screening Model to Evaluate Synthetic Cannabinoid MAM2201-Induced Effects on CNS.International journal of molecular sciences · 2023Article
- Polypropylene nanoplastic exposure leads to lung inflammation through p38-mediated NF-κB pathway due to mitochondrial damage.Particle and fibre toxicology · 2023Article
- Unraveling the potential human health risks from used disposable face mask-derived micro/nanoplastics during the COVID-19 pandemic scenario: A critical review.Environment international · 2022Review
- Micro- and Nanoplastics' Effects on Protein Folding and Amyloidosis.International journal of molecular sciences · 2022Review
- PS-NPs Induced Neurotoxic Effects in SHSY-5Y Cells via Autophagy Activation and Mitochondrial Dysfunction.Brain sciences · 2022Article
- Microplastics and Their Impact on Reproduction-Can we Learn From theFrontiers in toxicology · 2022Review
- Nanoplastic Impact on the Gut-Brain Axis: Current Knowledge and Future Directions.International journal of molecular sciences · 2021Review
- Characterizing the Role of Biologically Relevant Fluid Dynamics on Silver Nanoparticle Dependent Oxidative Stress in Adherent and Suspension In Vitro Models.Antioxidants (Basel, Switzerland) · 2021Article
- Newly Emerging Airborne Pollutants: Current Knowledge of Health Impact of Micro and Nanoplastics.International journal of environmental research and public health · 2021Review
- Neurodevelopmental Toxicity of Polystyrene Nanoplastics inACS omega · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanoparticles (NPs) have been shown to accumulate in organs, cross the blood-brain barrier and placenta, and have the potential to elicit developmental neurotoxicity (DNT). Here, we developed a human embryonic stem cell (hESC)-derived 3-dimensional (3-D) in vitro model that allows for testing of potential developmental neurotoxicants. Early central nervous system PAX6(+) precursor cells were generated from hESCs and differentiated further within 3-D structures. The 3-D model was characterized for neural marker expression revealing robust differentiation toward neuronal precursor cells, and gene expression profiling suggested a predominantly forebrain-like development. Altered neural gene expression due to exposure to non-cytotoxic concentrations of the known developmental neurotoxicant, methylmercury, indicated that the 3-D model could detect DNT. To test for specific toxicity of NPs, chemically inert polyethylene NPs (PE-NPs) were chosen. They penetrated deep into the 3-D structures and impacted gene expression at non-cytotoxic concentrations. NOTCH pathway genes such as HES5 and NOTCH1 were reduced in expression, as well as downstream neuronal precursor genes such as NEUROD1 and ASCL1. FOXG1, a patterning marker, was also reduced. As loss of function of these genes results in severe nervous system impairments in mice, our data suggest that the 3-D hESC-derived model could be used to test for Nano-DNT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.