ArticleInternational journal of molecular sciences2012
Simple estimation of Förster Resonance Energy Transfer (FRET) orientation factor distribution in membranes.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Lipid network crosslinked hydrogels control material dynamics across multiple length scales through lipid movement.Nature communications · 2026Article
- A synergistic dual-mode biosensor for methotrexate detection leveraging FRET and Ag NP-based UV absorption.RSC advances · 2026Article
- Integrating Molecular Dynamics Simulations and Single-molecule FRET Spectroscopy: From Computational FRET Estimation to Experimental Data Interpretation.The journal of physical chemistry. B · 2026Review
- Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Activatable Photosensitizers: From Fundamental Principles to Advanced Designs.Angewandte Chemie (International ed. in English) · 2025Review
- Interaction of the Antibiotic Rifampicin with Lipid Membranes.Biomolecules · 2025Article
- Evidence for a transfer-to-trap mechanism of fluorophore concentration quenching in lipid bilayers.Biophysical journal · 2024Article
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- Exploring safe and potent bioactives for the treatment of non-small cell lung cancer.3 Biotech · 2021Article
- The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations.Molecules (Basel, Switzerland) · 2020Review
- Sensitive detection of methylated DNA and methyltransferase activity based on the lighting up of FAM-labeled DNA quenched fluorescence by gold nanoparticles.RSC advances · 2019Article
- Homo- and hetero-oligomerization of hydrophobic pulmonary surfactant proteins SP-B and SP-C in surfactant phospholipid membranes.The Journal of biological chemistry · 2018Article
- Energy transfer and correlations in cavity-embedded donor-acceptor configurations.Scientific reports · 2018Article
- GPI-anchored proteins are confined in subdiffraction clusters at the apical surface of polarized epithelial cells.The Biochemical journal · 2017Article
- Computational refinement of spectroscopic FRET measurements.Data in brief · 2017Article
- An intermolecular FRET sensor detects the dynamics of T cell receptor clustering.Nature communications · 2017Article
- Structural rearrangement of the intracellular domains during AMPA receptor activation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane.The Journal of general physiology · 2016Article
- Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET.The Journal of general physiology · 2016Article
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Abstract
Because of its acute sensitivity to distance in the nanometer scale, Förster resonance energy transfer (FRET) has found a large variety of applications in many fields of chemistry, physics, and biology. One important issue regarding the correct usage of FRET is its dependence on the donor-acceptor relative orientation, expressed as the orientation factor k(2). Different donor/acceptor conformations can lead to k(2) values in the 0 ≤ k(2) ≤ 4 range. Because the characteristic distance for FRET, R(0), is proportional to (k(2))1/6, uncertainties in the orientation factor are reflected in the quality of information that can be retrieved from a FRET experiment. In most cases, the average value of k(2) corresponding to the dynamic isotropic limit (<k(2)> = 2/3) is used for computation of R(0) and hence donor-acceptor distances and acceptor concentrations. However, this can lead to significant error in unfavorable cases. This issue is more critical in membrane systems, because of their intrinsically anisotropic nature and their reduced fluidity in comparison to most common solvents. Here, a simple numerical simulation method for estimation of the probability density function of k(2) for membrane-embedded donor and acceptor fluorophores in the dynamic regime is presented. In the simplest form, the proposed procedure uses as input the most probable orientations of the donor and acceptor transition dipoles, obtained by experimental (including linear dichroism) or theoretical (such as molecular dynamics simulation) techniques. Optionally, information about the widths of the donor and/or acceptor angular distributions may be incorporated. The methodology is illustrated for special limiting cases and common membrane FRET pairs.
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