ArticleInternational journal of molecular sciences2012
Endpoint visual detection of three genetically modified rice events by loop-mediated isothermal amplification.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test.Molecular biology reports · 2025Article
- A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases ofHorticulture research · 2025Article
- A Colorimetric RT-LAMP Assay for Rapid Detection ofACS omega · 2024Article
- An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a.Foods (Basel, Switzerland) · 2023Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- DNA-Based Tools to Certify Authenticity of Rice Varieties-An Overview.Foods (Basel, Switzerland) · 2022Review
- Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology.Plants (Basel, Switzerland) · 2020Review
- Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART).Scientific reports · 2018Article
- A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA.Parasites & vectors · 2017Article
- Review
- Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection.Frontiers in plant science · 2017Review
- Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review.Frontiers in plant science · 2017Review
- Perspectives on genetically modified crops and food detection.Journal of food and drug analysis · 2016Review
- Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.Frontiers in plant science · 2016Review
- Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay.Frontiers in plant science · 2016Article
- Current and new approaches in GMO detection: challenges and solutions.BioMed research international · 2015Review
- Development and application of loop-mediated isothermal amplification assays for rapid visual detection of cry2Ab and cry3A genes in genetically-modified crops.International journal of molecular sciences · 2014Article
- New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination.MethodsX · 2014Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically modified (GM) rice KMD1, TT51-1, and KF6 are three of the most well known transgenic Bt rice lines in China. A rapid and sensitive molecular assay for risk assessment of GM rice is needed. Polymerase chain reaction (PCR), currently the most common method for detecting genetically modified organisms, requires temperature cycling and relatively complex procedures. Here we developed a visual and rapid loop-mediated isothermal amplification (LAMP) method to amplify three GM rice event-specific junction sequences. Target DNA was amplified and visualized by two indicators (SYBR green or hydroxy naphthol blue [HNB]) within 60 min at an isothermal temperature of 63 °C. Different kinds of plants were selected to ensure the specificity of detection and the results of the non-target samples were negative, indicating that the primer sets for the three GM rice varieties had good levels of specificity. The sensitivity of LAMP, with detection limits at low concentration levels (0.01%−0.005% GM), was 10- to 100-fold greater than that of conventional PCR. Additionally, the LAMP assay coupled with an indicator (SYBR green or HNB) facilitated analysis. These findings revealed that the rapid detection method was suitable as a simple field-based test to determine the status of GM crops.
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