ArticleInternational journal of molecular sciences2012
Selection of suitable reference genes for normalization of quantitative real-time PCR in cartilage tissue injury and repair in rabbits.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- WNT16 Regulation of the Articular Chondrocyte Phenotype in Mice.Life (Basel, Switzerland) · 2023Article
- Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.BMC zoology · 2022Article
- Computational Approach Reveals Pronociceptive Potential of Cannabidiol in Osteoarthritis: Role of Transient Receptor Potential Channels.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair.Journal of molecular histology · 2021Article
- Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system.BMC developmental biology · 2019Article
- Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model.Bone & joint research · 2019Article
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- Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis.Bone reports · 2018Article
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- A Tunable, Three-Dimensional In Vitro Culture Model of Growth Plate Cartilage Using Alginate Hydrogel Scaffolds.Tissue engineering. Part A · 2018Article
- [Effects of microRNA-140 gene transfection with nucleus localization signal linked nucleic kinase substrate short peptide conjugated chitosan on rabbit articular chondrocytes].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Article
- Gold nanoparticles approach to detect chondroitin sulphate and hyaluronic acid urothelial coating.Scientific reports · 2017Article
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When studying the altered expression of genes associated with cartilage regeneration by quantitative real-time RT-PCR (RT-qPCR), reference genes with highly stable expression during different stages of chondrocyte developmental are necessary to normalize gene expression accurately. Until now, no reports evaluating expression changes of commonly used reference genes in rabbit articular cartilage have been published. In this study, defects were made in rabbit articular cartilage, with or without insulin-like growth factor 1 (IGF-1) treatment, to create different chondrocyte living environments. The stability and intensity of the expressions of the candidate reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S Ribosomal RNA (18S rRNA), cyclophilin (CYP), hypoxanthine phosphoribosyl transferase (HPRT1), and beta-2-microglobulin (B2M) were evaluated. The data were analyzed by geNorm and NormFinder. B2M and 18S rRNA were identified to be suitable reference genes for rabbit cartilage tissues.
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