ArticleViruses2012
Insights into the functions of a prophage recombination directionality factor.
Article in Viruses, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix-turn-helix protein RdfS.Nucleic acids research · 2025Article
- Combining CRISPR/Cas9 and natural excision for the precise and complete removal of mobile genetic elements in bacteria.Applied and environmental microbiology · 2024Article
- Disarm The Bacteria: What Temperate Phages Can Do.Current issues in molecular biology · 2023Review
- Genetic Mining of Newly Isolated Salmophages for Phage Therapy.International journal of molecular sciences · 2022Article
- Phenotypic characterization and genome analysis of a novel Salmonella Typhimurium phage having unique tail fiber genes.Scientific reports · 2022Article
- Bioinformatic and experimental characterization of SEN1998: a conserved gene carried by the Enterobacteriaceae-associated ROD21-like family of genomic islands.Scientific reports · 2022Article
- Toxigenic properties and stx phage characterization of Escherichia coli O157 isolated from animal sources in a developing country setting.BMC microbiology · 2018Article
- Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum.The ISME journal · 2016Article
- Pathogenicity Island Cross Talk Mediated by Recombination Directionality Factors Facilitates Excision from the Chromosome.Journal of bacteriology · 2015Article
- Genome-based identification of active prophage regions by next generation sequencing in Bacillus licheniformis DSM13.PloS one · 2015Article
- Temperate phages acquire DNA from defective prophages by relaxed homologous recombination: the role of Rad52-like recombinases.PLoS genetics · 2014Article
- Phage-host interactions during pseudolysogeny: Lessons from the Pid/dgo interaction.Bacteriophage · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombination directionality factors (RDFs), or excisionases, are essential players of prophage excisive recombination. Despite the essentially catalytic role of the integrase in both integrative and excisive recombination, RDFs are required to direct the reaction towards excision and to prevent re-integration of the prophage genome when entering a lytic cycle. KplE1, HK620 and numerous (pro)phages that integrate at the same site in enterobacteria genomes (such as the argW tRNA gene) all share a highly conserved recombination module. This module comprises the attL and attR recombination sites and the RDF and integrase genes. The KplE1 RDF was named TorI after its initial identification as a negative regulator of the tor operon. However, it was characterized as an essential factor of excisive recombination. In this study, we designed an extensive random mutagenesis protocol of the torI gene and identified key residues involved in both functions of the TorI protein. We show that, in addition to TorI-TorR protein-protein interaction, TorI interacts in solution with the IntS integrase. Moreover, in vitro, TorR and IntS appear to compete for TorI binding. Finally, our mutagenesis results suggest that the C-terminal part of the TorI protein is dedicated to protein-protein interactions with both proteins TorR and IntS.
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