Trial reportJournal of proteomics2013
Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics.
Trial report in Journal of proteomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 73 citations in OpenAlex.
- Bacteriostatic Mechanism of the Ethyl Acetate Extract from the Root ofVeterinary sciences · 2026Article
- Proteomic and Lipidomic Atlas of Gut-Associated Lymph and Venous Depots in Female Piglets.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- Biological Fluid Flows: Signaling Mediums for Circadian Timing.Journal of biological rhythms · 2025Review
- Multi-site Ultrasound-guided Fine Needle Aspiration to Study Cells and Soluble Factors From Human Lymph Nodes.Current protocols · 2024Article
- Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load.Cell reports · 2024Article
- Mitomycin C and its analog trigger cytotoxicity in MCF-7 and K562 cancer cells through the regulation of RAS and MAPK/ERK pathways.Chemico-biological interactions · 2024Article
- Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming.European journal of immunology · 2024Article
- Chronic alcohol feeding alters lymph and plasma proteome in a rodent model.Life sciences · 2023Article
- Nanotechnologies for Physiology-Informed Drug Delivery to the Lymphatic System.Annual review of biomedical engineering · 2023Review
- Low protease activity in B cell follicles promotes retention of intact antigens after immunization.Science (New York, N.Y.) · 2023Article
- Oxidative Stress-Mediated Antibacterial Activity of the Total Flavonoid Extracted from theVeterinary sciences · 2022Article
- Proteomics of REPLICANT perfusate detects changes in the metastatic lymph node microenvironment.NPJ breast cancer · 2021Article
- Beyond a Passive Conduit: Implications of Lymphatic Biology for Kidney Diseases.Journal of the American Society of Nephrology : JASN · 2020Review
- The application of proteomics in the diagnosis and treatment of bronchial asthma.Annals of translational medicine · 2020Review
- Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients.The Journal of experimental medicine · 2019Article
- Senescence cell-associated extracellular vesicles serve as osteoarthritis disease and therapeutic markers.JCI insight · 2019Article
- Contribution of the plasma and lymph Degradome and Peptidome to the MHC Ligandome.Immunogenetics · 2019Review
- The Antigen Processing and Presentation Machinery in Lymphatic Endothelial Cells.Frontiers in immunology · 2019Review
- In vitro model reveals a role for mechanical stretch in the remodeling response of lymphatic muscle cells.Microcirculation (New York, N.Y. : 1994) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
In this study a proteomic approach was used to define the protein content of matched samples of afferent prenodal lymph and plasma derived from healthy volunteers. The analysis was performed using two analytical methodologies coupled with nanoliquid chromatography-tandem mass spectrometry: one-dimensional gel electrophoresis (1DEF nanoLC Orbitrap-ESI-MS/MS), and two-dimensional fluorescence difference-in-gel electrophoresis (2D-DIGE nanoLC-ESI-MS/MS). The 253 significantly identified proteins (p<0.05), obtained from the tandem mass spectrometry data, were further analyzed with pathway analysis (IPA) to define the functional signature of prenodal lymph and matched plasma. The 1DEF coupled with nanoLC-MS-MS revealed that the common proteome between the two biological fluids (144 out of 253 proteins) was dominated by complement activation and blood coagulation components, transporters and protease inhibitors. The enriched proteome of human lymph (72 proteins) consisted of products derived from the extracellular matrix, apoptosis and cellular catabolism. In contrast, the enriched proteome of human plasma (37 proteins) consisted of soluble molecules of the coagulation system and cell-cell signaling factors. The functional networks associated with both common and source-distinctive proteomes highlight the principal biological activity of these immunologically relevant body fluids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.