ArticleBiochimica et biophysica acta2013
Lipid modification gives rise to two distinct Haloferax volcanii S-layer glycoprotein populations.
Article in Biochimica et biophysica acta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Two coinfecting archaeal viruses provide insights into virus-virus interactions.Communications biology · 2026Article
- Characterization of aliA and aliB deletion mutants reveals a dominant role of AliA in Haloferax volcanii lipoprotein lipidation.The Journal of biological chemistry · 2026Article
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Article
- A Micrarchaeon Isolate Is Covered by a Proteinaceous S-Layer.Applied and environmental microbiology · 2022Article
- Article
- Article
- Functional Prediction and Assignment ofFrontiers in genetics · 2020Article
- Archaeal cell surface biogenesis.FEMS microbiology reviews · 2018Article
- Sialic Acid-Like Sugars in Archaea: Legionaminic Acid Biosynthesis in the HalophileFrontiers in microbiology · 2018Article
- Archaeal S-Layers: Overview and Current State of the Art.Frontiers in microbiology · 2017Review
- Emerging facets of prokaryotic glycosylation.FEMS microbiology reviews · 2017Review
- Permuting the PGF Signature Motif Blocks both Archaeosortase-Dependent C-Terminal Cleavage and Prenyl Lipid Attachment for the Haloferax volcanii S-Layer Glycoprotein.Journal of bacteriology · 2015Article
- Archaeal membrane-associated proteases: insights on Haloferax volcanii and other haloarchaea.Frontiers in microbiology · 2015Review
- Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules.Journal of the Royal Society, Interface · 2014Review
- Archaeal S-layer glycoproteins: post-translational modification in the face of extremes.Frontiers in microbiology · 2014Article
- Bacterial cell-envelope glycoconjugates.Advances in carbohydrate chemistry and biochemistry · 2013Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The S-layer glycoprotein is the sole component of the protein shell surrounding Haloferax volcanii cells. The deduced amino acid sequence of the S-layer glycoprotein predicts the presence of a C-terminal membrane-spanning domain. However, several earlier observations, including the ability of EDTA to selectively solubilize the protein, are inconsistent with the presence of a trans-membrane sequence. In the present report, sequential solubilization of the S-layer glycoprotein by EDTA and then with detergent revealed the existence of two distinct populations of the S-layer glycoprotein. Whereas both S-layer glycoprotein populations underwent signal peptide cleavage and N-glycosylation, base hydrolysis followed by mass spectrometry revealed that a lipid, likely archaetidic acid, modified only the EDTA-solubilized version of the protein. These observations are consistent with the S-layer glycoprotein being initially synthesized as an integral membrane protein and subsequently undergoing a processing event in which the extracellular portion of the protein is separated from the membrane-spanning domain and transferred to a waiting lipid moiety.
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Registered trials
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