ArticleBiophysical journal2012
Phosphatidylserine inhibits and calcium promotes model membrane fusion.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- The Conserved Fusion Peptide Sequence of SARS-CoVs is Insensitive to CaThe Journal of membrane biology · 2026Article
- Membrane-Targeting Antivirals.International journal of molecular sciences · 2025Review
- Chimeric nanobody-decorated liposomes by self-assembly.Nature nanotechnology · 2024Article
- Lipid and Lipidation in Membrane Fusion.The Journal of membrane biology · 2022Review
- CaFaculty reviews · 2022Review
- Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation.Acta pharmaceutica Sinica. B · 2021Article
- Translation of Mycobacterium Survival Strategy to Develop a Lipo-peptide based Fusion Inhibitor*.Angewandte Chemie (International ed. in English) · 2021Article
- Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients.Journal of visualized experiments : JoVE · 2018Article
- Overexpression of Sly41 suppresses COPII vesicle-tethering deficiencies by elevating intracellular calcium levels.Molecular biology of the cell · 2016Article
- Phosphatidylserine-Dependent Catalysis of Stalk and Pore Formation by Synaptobrevin JMR-TMD Peptide.Biophysical journal · 2015Article
- Sec17 can trigger fusion of trans-SNARE paired membranes without Sec18.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Is Spontaneous Translocation of Polar Lipids Between Cellular Organelles Negligible?Lipid insights · 2015Review
- The transmembrane domain peptide of vesicular stomatitis virus promotes both intermediate and pore formation during PEG-mediated vesicle fusion.Biophysical journal · 2014Article
- pH Alters PEG-mediated fusion of phosphatidylethanolamine-containing vesicles.Biophysical journal · 2014Article
- Wild-type and mutant hemagglutinin fusion peptides alter bilayer structure as well as kinetics and activation thermodynamics of stalk and pore formation differently: mechanistic implications.Biophysical journal · 2013Article
- A novel assay for detecting fusion pore formation: implications for the fusion mechanism.Biochemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
PEG-mediated fusion of SUVs composed of dioleoylphosphatidylcholine, dioleoylphosphatidylethanolamine, sphingomyelin, cholesterol, and dioleoylphosphatidylserine was examined to investigate the effects of PS on the fusion mechanism. Lipid mixing, content mixing, and content leakage measurements were carried out with vesicles containing from 0 to 8 mol % PS and similar amounts of phosphatidylglycerol. Fitting these time courses globally to a 3-state (aggregate, intermediate, pore) sequential model established rate constants for each step and probabilities of lipid mixing, content mixing, and leakage in each state. Charged lipids inhibited both the rates of intermediate and pore formation as well as the extents of lipid and contents mixing, although electrostatics were not solely responsible for inhibition. Ca(2+) counteracted this inhibition and increased the extent of fusion in the presence of PS to well beyond that seen in the absence of charged lipids. The effects of both PS and Ca(2+) could be interpreted in terms of a previous proposal for the nature of lipid fluctuations that account for transition states for the two steps of the fusion process examined. The results suggest a more significant role for Ca(2+)-lipid interactions than is acknowledged in current thinking about cell membrane fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.