ArticleBiophysical journal2012
Importance of the sphingoid base length for the membrane properties of ceramides.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Comprehensive Isomer-Resolved Ganglioside Profiling by HILIC-nanoESI-MS/MS.Analytical chemistry · 2026Article
- Central Roles of Glucosylceramide in Driving Cancer Pathogenesis.International journal of molecular sciences · 2025Review
- Skin Lipid Barrier: Structure, Function and Metabolism.Allergy, asthma & immunology research · 2024Review
- Sphingolipids: Less Enigmatic but Still Many Questions about the Role(s) of Ceramide in the Synthesis/Function of the Ganglioside Class of Glycosphingolipids.International journal of molecular sciences · 2024Review
- COVID-19, Blood Lipid Changes, and Thrombosis.Biomedicines · 2023Review
- Lipid Self-Assemblies under the Atomic Force Microscope.International journal of molecular sciences · 2021Review
- Ceramide-containing liposomes with doxorubicin: time and cell-dependent effect of C6 and C12 ceramide.Oncotarget · 2017Article
- The Long-Chain Sphingoid Base of Ceramides Determines Their Propensity for Lateral Segregation.Biophysical journal · 2017Article
- Article
- Influence of Hydroxylation, Chain Length, and Chain Unsaturation on Bilayer Properties of Ceramides.Biophysical journal · 2015Article
- Trafficking and Functions of Bioactive Sphingolipids: Lessons from Cells and Model Membranes.Lipid insights · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The sphingoid bases of sphingolipids, including ceramides, can vary in length from 12 to >20 carbons. To study how such length variation affects the bilayer properties of ceramides, we synthesized ceramides consisting of a C12-, C14-, C16-, C18-, or C20-sphing-4-enin derivative coupled to palmitic acid. The ceramides were studied in mixtures with palmitoyloleoylphosphocholine (POPC) and/or palmitoylsphingomyelin (PSM), and in more complex bilayers also containing cholesterol. The trans-parinaric acid lifetimes showed that 12:1- and 14:1-PCer failed to increase the order of POPC bilayers, whereas 16:1-, 18:1-, and 20:1-PCer induced ordered- or gel-phase formation. Nevertheless, all of the analogs were able to thermally stabilize PSM, and a chain-length-dependent increase in the main phase transition temperature of equimolar PSM/Cer bilayers was revealed by differential scanning calorimetry. Similar thermal stabilization of PSM-rich domains by the ceramides was observed in POPC bilayers with a trans-parinaric acid-quenching assay. A cholestatrienol-quenching assay and sterol partitioning experiments showed that 18:1- and 20:1-PCer formed sterol-excluding gel phases with PSM, reducing the overall bilayer affinity of sterol. The effect of 16:1-PCer on sterol distribution was less dramatic, and no displacement of sterol from the PSM environment was observed with 12:1- and 14:1-PCer. The results are discussed in relation to other structural features that affect the bilayer properties of ceramides.
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