ArticleBiophysical journal2012
In vivo biochemistry in bacterial cells using FRAP: insight into the translation cycle.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Inferring diffusion, reaction, and exchange parameters from imperfect FRAP.Biophysical journal · 2025Article
- Differential growth regulates asymmetric size partitioning inLife science alliance · 2024Article
- Dependence of diffusion ineLife · 2022Article
- Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion.Journal of mathematical biology · 2021Article
- Article
- Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae.BMC biology · 2020Article
- The lipoprotein Pal stabilises the bacterial outer membrane during constriction by a mobilisation-and-capture mechanism.Nature communications · 2020Article
- Article
- Resolving Cytosolic Diffusive States in Bacteria by Single-Molecule Tracking.Biophysical journal · 2019Article
- A Highly Accurate Pixel-Based FRAP Model Based on Spectral-Domain Numerical Methods.Biophysical journal · 2019Article
- Understanding Protein Mobility in Bacteria by Tracking Single Molecules.Journal of molecular biology · 2018Review
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- Spatial organization and dynamics of RNase E and ribosomes inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- How Important Is Protein Diffusion in Prokaryotes?Frontiers in molecular biosciences · 2018Review
- Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter.The EMBO journal · 2017Article
- Spatial organization of bacterial transcription and translation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Slow Mobility of the ParA Partitioning Protein Underlies Its Steady-State Patterning in Caulobacter.Biophysical journal · 2016Article
- Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells.Nature communications · 2016Article
- Localization of the outer membrane protein OmpA2 in Caulobacter crescentus depends on the position of the gene in the chromosome.Journal of bacteriology · 2014Article
- The bacterial cytoplasm has glass-like properties and is fluidized by metabolic activity.Cell · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In vivo measurements of the mobility and binding kinetics of cellular components are essential to fully understand the biochemical processes occurring inside cells. Here, we describe a fluorescence recovery after photobleaching-based method that can be easily implemented to the study of reaction-diffusion processes in live bacteria despite their small size. We apply this method to provide new, to our knowledge, quantitative insight into multiple aspects of the bacterial translation cycle by measuring the binding kinetics and the micrometer-scale diffusive properties of the 50S ribosomal subunit in live Caulobacter cells. From our measurements, we infer that 70% of 50S subunits are engaged in translation and display, on average, limited motion on the micrometer scale, consistent with little mixing of transcripts undergoing translation. We also extract the average rate constants for the binding of 50S subunits to 30S initiation complexes during initiation and for their release from mRNAs when translation is completed. From this, we estimate the average time of protein synthesis and the average search time of 50S subunits before they engage in the next initiation event. Additionally, our experiments suggest that so-called free 50S subunits do not diffuse freely; instead their mobility is significantly slowed down, possibly through transient associations with mRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.