ArticleBosnian journal of basic medical sciences2012
Trefoil factor 3 isolated from human breast milk downregulates cytokines (IL8 and IL6) and promotes human beta defensin (hBD2 and hBD4) expression in intestinal epithelial cells HT-29.
Article in Bosnian journal of basic medical sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Does the Alpha-defensin Immunoassay or the Lateral Flow Test Have Better Diagnostic Value for Periprosthetic Joint Infection? A Meta-analysis.Clinical orthopaedics and related research · 2018Pooled it
- Intestinal mucus components and secretion mechanisms: what we do and do not know.Experimental & molecular medicine · 2023Review
- Effects of human donor milk on gut barrier function and inflammation:Frontiers in immunology · 2023Article
- Tff3 Deficiency Protects against Hepatic Fat Accumulation after Prolonged High-Fat Diet.Life (Basel, Switzerland) · 2022Article
- Pathological and therapeutic roles of bioactive peptide trefoil factor 3 in diverse diseases: recent progress and perspective.Cell death & disease · 2022Review
- Peptides Derived from In Vitro and In Vivo Digestion of Human Milk Are Immunomodulatory in THP-1 Human Macrophages.The Journal of nutrition · 2022Article
- Maternal vaccination as an additional approach to improve the protection of the nursling: Anti-infective properties of breast milk.Clinics (Sao Paulo, Brazil) · 2022Review
- Chemical Synthesis of TFF3 Reveals Novel Mechanistic Insights and a Gut-Stable Metabolite.Journal of medicinal chemistry · 2021Article
- LINGO3 regulates mucosal tissue regeneration and promotes TFF2 dependent recovery from colitis.Scandinavian journal of gastroenterology · 2021Article
- Structure, Function, and Therapeutic Potential of the Trefoil Factor Family in the Gastrointestinal Tract.ACS pharmacology & translational science · 2020Review
- Impact of Holder pasteurization on immunological properties of human breast milk over the first year of lactation.Pediatric research · 2020Article
- TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths.Nature communications · 2019Article
- Prolonged Suppressive Effects of Periodontitis on Salivary TFF3 Production.European journal of dentistry · 2019Article
- Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China.Clinical proteomics · 2019Article
- Effect of Initial Periodontal Therapy on Salivary Trefoil Factor (TFF3) in otherwise Healthy Patients with Gingivitis and Chronic Periodontitis.Contemporary clinical dentistry · 2018Article
- Localization of trefoil factor family peptide 3 (TFF3) in epithelial tissues originating from the three germ layers of developing mouse embryo.Bosnian journal of basic medical sciences · 2017Article
- Trefoil Factor Peptides and Gastrointestinal Function.Annual review of physiology · 2017Review
- Human Trefoil Factor 3 induces the transcription of its own promoter through STAT3.Scientific reports · 2016Article
- LPS preconditioning ameliorates intestinal injury in a rat model of hemorrhagic shock.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trefoil factors (TFF) are secretory products of mucin producing cells. They play a key role in the maintenance of the surface integrity of oral mucosa and enhance healing of the gastrointestinal mucosa by a process called restitution. TFF comprises the gastric peptides (TFF1), spasmolytic peptide (TFF2), and the intestinal trefoil factor (TFF3). They have an important and necessary role in epithelial restitution within the gastrointestinal tract. Significant amounts of TFF are present in human milk. This study aimed to determine a possible correlation between TFF3 isolated from human breast milk and levels of cytokines (IL8 and IL6) and defensins (hBD2 and hBD4) in intestinal epithelial cells HT-29 treated with trefoil. Samples of human milk were collected within 2-4 weeks postpartum from healthy human mothers (18-30-years-old) by manual breast massage, and TFF3 was purified by ammonium sulfate precipitation, isoelectric precipitation, DEAE-chromatography, and gel filtration. In this work we measured the concentrations and mRNA levels of cytokines and defensins by immunoassay (ELISA) and semiquantitative RT-PCR technique, respectively. Also we measured the peroxidase activity. We present the first evidence of human milk TFF3 purification. Here we show that the presence of TFF3 isolated from milk strongly correlates with downregulation of IL8 and IL6 in human intestinal epithelial cells. On the other hand, TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 (hBD4). These findings suggest that TFF can activate intestinal epithelial cells and could actively participate in the immune system of breastfed babies by inducing the production of peptides related to innate defence, such as defensins.
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