ArticleJournal of ophthalmic & vision research2009
Retinal pigment epithelium culture;a potential source of retinal stem cells.
Article in Journal of ophthalmic & vision research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- The effect of enhancers on the lentiviral transduction efficiency in the human RPE cells: Insights for advancing retinal gene therapies.Biochemistry and biophysics reports · 2025Article
- Review
- Comprehensive Analysis of Photoreceptor Outer Segments: Flow Cytometry Characterization and Stress-Driven Impact on Retinal Pigment Epithelium Phagocytosis.International journal of molecular sciences · 2023Article
- Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium.International journal of molecular sciences · 2022Article
- Evaluation of the Potential Effects of Retinol and Alginate/Gelatin-Based Scaffolds on Differentiation Capacity of Mouse Mesenchymal Stem Cells (MSCs) into Retinal Cells.International journal of stem cells · 2022Article
- Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells.Iranian journal of basic medical sciences · 2019Article
- Primary Cell Cultures from the Mouse Retinal Pigment Epithelium.Journal of visualized experiments : JoVE · 2018Article
- Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side.The Journal of biological chemistry · 2017Article
- Tissue Engineering and Regenerative Medicine in Iran: Current State of Research and Future Outlook.Molecular biotechnology · 2015Review
- Amniotic fluid promotes the appearance of neural retinal progenitors and neurons in human RPE cell cultures.Molecular vision · 2013Article
- Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid.BMC research notes · 2012Article
- RPE-secreted factors: influence differentiation in human retinal cell line in dose- and density-dependent manner.Journal of ocular biology, diseases, and informatics · 2010Article
- Ocular Drug Delivery; Impact of in vitro Cell Culture Models.Journal of ophthalmic & vision research · 2009Article
- Retinal pigment epithelium derived stem cells.Journal of ophthalmic & vision research · 2009Article
- Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations.Journal of tissue engineeringReview
- Effect of Pulsed Low-Level Lasers on Adult versus Neonatal Human Retinal Pigment Epithelial Cells: AnJournal of ophthalmic & vision researchArticle
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo establish human retinal pigment epithelial (RPE) cell culture as a source for cell replacement therapy in ocular diseases.
methodsHuman cadaver globes were used to isolate RPE cells. Each globe was cut into several pieces of a few millimeters in size. After removing the sclera and choroid, remaining tissues were washed in phosphate buffer saline and RPE cells were isolated using dispase enzyme solution and cultured in Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 supplemented with 10% fetal calf serum.
resultsPrimary cultures of RPE cells were established and spheroid colonies related to progenitor/stem cells developed in a number of cultures. The colonies included purely pigmented or mixed pigmented and non-pigmented cells. After multiple cellular passages, several types of photoreceptors and neural-like cells were detected morphologically.
conclusionCellular plasticity in RPE cell cultures revealed promising results in terms of generation of stem/progenitor cells from human RPE cells. Whether the spheroids and neural-like retinal cells were directly derived from retinal stem cells or offspring of trans-differentiating or de-differentiating RPE cells remains to be answered.
Indexed as
Identifiers
23198062PMC3498558W2129171040What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.