ArticleNature protocols2012
Monitoring local synaptic activity with astrocytic patch pipettes.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Astrocytic mGluR5 Regulation of Synaptic Transmission is Activity-Dependent in Adult Rats.Glia · 2026Article
- Differential Astrocyte-Supplied NMDAR Co-Agonist for CA1 Versus Dentate Gyrus Long-Term Potentiation.Hippocampus · 2025Article
- Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain.Cell reports · 2025Article
- Article
- Astrocyte β-Adrenergic Receptor Activity Regulates NMDA Receptor Signaling of Medial Prefrontal Cortex Pyramidal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Astrocyte's self-repairing characteristics improve working memory in spiking neuronal networks.iScience · 2023Article
- Non-trivial dynamics in a model of glial membrane voltage driven by open potassium pores.Biophysical journal · 2023Article
- Article
- On the electrical passivity of astrocyte potassium conductance.Journal of neurophysiology · 2021Review
- Heterogeneity and Development of Fine Astrocyte Morphology Captured by Diffraction-Limited Microscopy.Frontiers in cellular neuroscience · 2021Article
- LTP Induction Boosts Glutamate Spillover by Driving Withdrawal of Perisynaptic Astroglia.Neuron · 2020Article
- Making sense of astrocytic calcium signals - from acquisition to interpretation.Nature reviews. Neuroscience · 2020Review
- Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contributes to synaptic alterations in nucleus tractus solitarii.Journal of neurophysiology · 2020Article
- Stress gates an astrocytic energy reservoir to impair synaptic plasticity.Nature communications · 2020Article
- Local Resting CaCell reports · 2020Article
- Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii.American journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Reducing Glutamate Uptake in Rat Hippocampal Slices Enhances Astrocytic Membrane Depolarization While Down-Regulating CA3-CA1 Synaptic Response.Frontiers in synaptic neuroscience · 2020Article
- Spontaneous Ultraslow NaCells · 2019Article
- Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo.Scientific reports · 2017Article
- Dopamine elevates and lowers astroglial CaGlia · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Rapid signal exchange between astroglia and neurons has emerged as a key player in neural communication in the brain. To understand the mechanisms involved, it is often important to have access to individual astrocytes while monitoring the activity of nearby synapses. Achieving this with standard electrophysiological tools is not always feasible. The protocol presented here enables the monitoring of synaptic activity using whole-cell current-clamp recordings from a local astrocyte. This approach takes advantage of the fact that the low input resistance of electrically passive astroglia allows extracellular currents to pass through the astrocytic membrane with relatively little attenuation. Once the slice preparation is ready, it takes ~30 min to several hours to implement this protocol, depending on the experimental design, which is similar to other patch-clamp techniques. The technique presented here can be used to directly access the intracellular medium of individual astrocytes while examining synapses functioning in their immediate proximity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.