ArticlemAbs
Adsorption behavior of a human monoclonal antibody at hydrophilic and hydrophobic surfaces.
Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 68 citations in OpenAlex.
- A Rational Optimization Approach for the Development of a Multiplexed Lateral Flow Immunoassay: Detection of Nonepithelial Ovarian Cancer Markers in Human Serum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Development of a Piezoelectric Immunosensor to Rapidly Measure Prohibitin‑1 as a Prognostic Biomarker for Sepsis and Other Serious Infectious Diseases.ACS applied electronic materials · 2025Article
- pH-Dependent Conformational Plasticity of Monoclonal Antibodies at the SiOACS applied materials & interfaces · 2024Article
- Mechanistic Insights into the Adsorption of Monoclonal Antibodies at the Water/Vapor Interface.Molecular pharmaceutics · 2024Article
- Competitive Adsorption of a Monoclonal Antibody and Nonionic Surfactant at the PDMS/Water Interface.Molecular pharmaceutics · 2023Article
- A Quick and Reproducible Silanization Method by Using Plasma Activation for Hydrophobicity-Based Kinesin Single Molecule Fluorescence-Microscopy Assays.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Machine Learning Analysis Provides Insight into Mechanisms of Protein Particle Formation Inside Containers During Mechanical Agitation.Journal of pharmaceutical sciences · 2022Article
- Inhaled antibodies: Quality and performance considerations.Human vaccines & immunotherapeutics · 2022Review
- Surfactant Protection Efficacy at Surfaces Varies with the Nature of Hydrophobic Materials.Pharmaceutical research · 2021Article
- Nanomaterials-Based Sensors for Respiratory Viral Detection: A Review.IEEE sensors journal · 2021Article
- A Narrative Review of a Pulmonary Aerosolized Formulation or a Nasal Drop Using Sera Containing Neutralizing Antibodies Collected from COVID-19-Recovered Patients as a Probable Therapy for COVID-19.Iranian journal of medical sciences · 2021Review
- Adsorption of non-ionic surfactant and monoclonal antibody on siliconized surface studied by neutron reflectometry.Journal of colloid and interface science · 2021Article
- Recent Advances in Studying Interfacial Adsorption of Bioengineered Monoclonal Antibodies.Molecules (Basel, Switzerland) · 2020Review
- Evaluation of Crystal Zenith Microtiter Plates for High-Throughput Formulation Screening.Journal of pharmaceutical sciences · 2020Article
- Observation of high-temperature macromolecular confinement in lyophilised protein formulations using terahertz spectroscopy.International journal of pharmaceutics: X · 2019Article
- Fluctuations in Pharmacokinetics Profiles of Monoclonal Antibodies.European journal of drug metabolism and pharmacokinetics · 2019Article
- Oriented attachment of VChemical communications (Cambridge, England) · 2019Article
- Neutron reflectivity measurement of protein A-antibody complex at the solid-liquid interface.Journal of chromatography. A · 2017Article
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Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One aspiration for the formulation of human monoclonal antibodies (mAb) is to reach high solution concentrations without compromising stability. Protein surface activity leading to instability is well known, but our understanding of mAb adsorption to the solid-liquid interface in relevant pH and surfactant conditions is incomplete. To investigate these conditions, we used total internal reflection fluorescence (TIRF) and neutron reflectometry (NR). The mAb tested ("mAb-1") showed highest surface loading to silica at pH 7.4 (~12 mg/m(2)), with lower surface loading at pH 5.5 (~5.5 mg/m(2), further from its pI of 8.99) and to hydrophobized silica (~2 mg/m(2)). The extent of desorption of mAb-1 from silica or hydrophobized silica was related to the relative affinity of polysorbate 20 or 80 for the same surface. mAb-1 adsorbed to silica on co-injection with polysorbate (above its critical micelle concentration) and also to silica pre-coated with polysorbate. A bilayer model was developed from NR data for mAb-1 at concentrations of 50-5000 mg/L, pH 5.5, and 50-2000 mg/L, pH 7.4. The inner mAb-1 layer was adsorbed to the SiO₂ surface at near saturation with an end-on" orientation, while the outer mAb-1 layer was sparse and molecules had a "side-on" orientation. A non-uniform triple layer was observed at 5000 mg/L, pH 7.4, suggesting mAb-1 adsorbed to the SiO₂ surface as oligomers at this concentration and pH. mAb-1 adsorbed as a sparse monolayer to hydrophobized silica, with a layer thickness increasing with bulk concentration - suggesting a near end-on orientation without observable relaxation-unfolding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.