ArticlePediatric surgery international2013
"Trooping the color": restoring the original donor skin color by addition of melanocytes to bioengineered skin analogs.
Article in Pediatric surgery international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Anatomy of a bioengineered human pigmented skin equivalent to provide fundamental insights into skin tone melanin dynamics.Journal of anatomy · 2026Article
- Safety and efficacy of bio-engineered, autologous dermo-epidermal skin grafts in adolescent and adult burn patients: 1-year results of a prospective, randomized, controlled, multicenter phase IIB clinical trial.EClinicalMedicine · 2025Article
- Laboratory-Generated Autologous Skin Substitutes for Burn Treatment in Europe: Narrative Review, Experts' Opinion, and Legal Considerations.European burn journal · 2025Review
- Long-Term Outcomes of a Cultured Autologous Dermo-Epidermal Skin Substitute in Children: 5-Year Results of a Phase I Clinical Trial.Journal of burn care & research : official publication of the American Burn Association · 2025Article
- Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Isolation and Culture of Human Dermal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Combining bioengineered human skin with bioprinted cartilage for ear reconstruction.Science advances · 2023Article
- Article
- Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review).Biomedicines · 2022Review
- A Simple Method for the Production of Human Skin Equivalent in 3D, Multi-Cell Culture.International journal of molecular sciences · 2020Article
- The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds.Stem cells international · 2020Review
- Induction of angiogenic and inflammation-associated dermal biomarkers following acute UVB exposure on bio-engineered pigmented dermo-epidermal skin substitutes in vivo.Pediatric surgery international · 2019Article
- Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes.Pediatric surgery international · 2019Article
- Advances in keratinocyte delivery in burn wound care.Advanced drug delivery reviews · 2018Review
- Tissue engineering of skin and regenerative medicine for wound care.Burns & trauma · 2018Review
- Review
- Comparison of in vivo immune responses following transplantation of vascularized and non-vascularized human dermo-epidermal skin substitutes.Pediatric surgery international · 2017Article
- Skin Tissue Engineering: Application of Adipose-Derived Stem Cells.BioMed research international · 2017Review
- Myelinated and unmyelinated nerve fibers reinnervate tissue-engineered dermo-epidermal human skin analogs in an in vivo model.Pediatric surgery international · 2016Article
- Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute.Pediatric surgery international · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeAutologous skin substitutes to cover large skin defects are used since several years. Melanocytes, although essential for solar protection and pigmentation of skin, are not yet systematically added to such substitutes. In this experimental study, we reconstructed melanocyte-containing dermo-epidermal skin substitutes from donor skins of different skin pigmentation types and studied them in an animal model. Features pertinent to skin color were analyzed and compared in both skin substitutes and original donor skin.
methodsKeratinocytes, melanocytes, and fibroblast were isolated, cultured, and expanded from skin biopsies of light- and dark-pigmented patients. For each donor, melanocytes and keratinocytes were seeded in different ratios (1:1, 1:5, 1:10) onto collagen gels previously populated with autologous fibroblasts. Skin substitutes were then transplanted onto full-thickness wounds of immuno-incompetent rats. After 8 weeks, macroscopic and microscopic analyses were conducted with regard to skin color and architecture.
resultsChromameter evaluation revealed that skin color of reconstructed light- and dark-pigmented skin was very similar to donor skin, independent of which melanocyte/keratinocyte ratio was added. Histological analyses of the skin analogs confirmed these findings.
conclusionThese data suggest that adding autologous melanocytes to bioengineered dermo-epidermal skin analogs can sustainably restore the patients' native skin color.
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