Evidence map›Paper›PMID 23196068›Full record

ArticleNeuropharmacology2013

L-dopa-induced dopamine synthesis and oxidative stress in serotonergic cells.

Branden J Stansley, Bryan K Yamamoto

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 103 citations in OpenAlex.

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  18. Pharmaceutical biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Branden J StansleyDepartment of Neurosciences, University of Toledo, College of Medicine, 3000 Arlington Avenue, Mail Stop 1007, Toledo, OH 43614, USA.
Bryan K Yamamoto
University of Toledo · US

Funding

METHAMPHETAMINE TOXICITY AND CORTICOSTRIATAL GLUTAMATER01DA007606 · NIDA · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI YAMAMOTO, BRYAN K · 1992 to 2016
$5.1M
Role of Tyrosine in MDMA ToxicityR01DA019486 · NIDA · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI YAMAMOTO, BRYAN K · 2006 to 2010
$1.3M
NIDA NIH HHS DA019486NIDA NIH HHS R01 DA007606NIDA NIH HHS R01 DA019486
6 · The paper itself

Abstract

L-dopa is a precursor for dopamine synthesis and a mainstay treatment for Parkinson's disease. However, l-dopa therapy is not without side effects that may be attributed to non-dopaminergic mechanisms. Synthesized dopamine can be neurotoxic through its enzymatic degradation by monoamine oxidase (MAO) to form the reactive byproduct, hydrogen peroxide and hydroxyl radicals or through auto-oxidation to form highly reactive quinones that can bind proteins and render them non-functional. Since l-dopa could be decarboxylated by aromatic amino acid decarboxylase (AADC) present within both dopamine and serotonin neurons, it was hypothesized that serotonin neurons convert l-dopa into dopamine to generate excessive reactive oxygen species and quinoproteins that ultimately lead to serotonin neuron death. To examine the effects of l-dopa on serotonin neurons, the RN46A-B14 cell line was used. These immortalized serotonergic cell cultures were terminally differentiated and then incubated with varying concentrations of l-dopa. Results show that RN46A-B14 cells contain AADC and can synthesize dopamine after incubation with l-dopa. Furthermore, l-dopa dose-dependently increased intracellular reactive oxygen species (ROS) and cell death. Dopamine, ROS production and cell death were attenuated by co-incubation with the AADC inhibitor, NSD-1015. The MAO inhibitor, pargyline, also attenuated cell death and ROS after l-dopa treatment. Lastly, quinoprotein formation was enhanced significantly by incubation with l-dopa. Taken together, these data illustrate that serotonergic cells can produce dopamine and that the accumulation of dopamine after l-dopa and its subsequent degradation can lead to ROS production and death of RN46A-B14 serotonergic cells.

Indexed as

Cell Line, TransformedDopamineHumansLevodopaOxidative StressReactive Oxygen SpeciesSerotonergic NeuronsDopamineLevodopaReactive Oxygen Species

Identifiers

PMID23196068
PMCPMC3638241
OpenAlexW1988146449

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.