ArticleJournal of the American Chemical Society2012
A logical molecular circuit for programmable and autonomous regulation of protein activity using DNA aptamer-protein interactions.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 124 citations in OpenAlex.
- Hierarchical core-shell DNA condensates enable programmable information storage and encryption.Nature communications · 2025Article
- Synthetic CRISPR Networks Driven by Transcription Factors via Structure-Switching DNA Translators.Journal of the American Chemical Society · 2025Article
- Photochemically Triggered, Transient, and Oscillatory Transcription Machineries Guide Temporal Modulation of Fibrinogenesis.Journal of the American Chemical Society · 2025Article
- Aptamer-Based DNA Allosteric Switch for Regulation of Protein Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Targeted degradation of VEGF with bispecific aptamer-based LYTACs ameliorates pathological retinal angiogenesis.Theranostics · 2024Article
- Structure and dynamics of an archetypal DNA nanoarchitecture revealed via cryo-EM and molecular dynamics simulations.Nature communications · 2023Article
- Using CIVT-SELEX to Select Aptamers as Genetic Parts to Regulate Gene Circuits in a Cell-Free System.International journal of molecular sciences · 2023Article
- DNA-based customized functional modules for signal transformation.Frontiers in chemistry · 2023Review
- Network topology-directed design of molecular CPU for cell-like dynamic information processing.Science advances · 2022Article
- A kinetically controlled platform for ligand-oligonucleotide transduction.Nature communications · 2021Article
- Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Propelling DNA Computing with Materials' Power: Recent Advancements in Innovative DNA Logic Computing Systems and Smart Bio-Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Logic Gates Based on DNA Aptamers.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Modular protein-oligonucleotide signal exchange.Nucleic acids research · 2020Article
- Dynamic colloidal nanoparticle assembly triggered by aptamer-receptor interactions on live cell membranes.Chemical science · 2019Article
- Designed and Evolved Nucleic Acid Nanotechnology: Contrast and Complementarity.Bioconjugate chemistry · 2019Review
- DNA-Based Dynamic Reaction Networks.Trends in biochemical sciences · 2018Review
- Constructing Smart Protocells with Built-In DNA Computational Core to Eliminate Exogenous Challenge.Journal of the American Chemical Society · 2018Article
- Hierarchical control of enzymatic actuators using DNA-based switchable memories.Nature communications · 2017Article
- Engineered Aptamers to Probe Molecular Interactions on the Cell Surface.Biomedicines · 2017Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Researchers increasingly envision an important role for artificial biochemical circuits in biological engineering, much like electrical circuits in electrical engineering. Similar to electrical circuits, which control electromechanical devices, biochemical circuits could be utilized as a type of servomechanism to control nanodevices in vitro, monitor chemical reactions in situ, or regulate gene expressions in vivo. (1) As a consequence of their relative robustness and potential applicability for controlling a wide range of in vitro chemistries, synthetic cell-free biochemical circuits promise to be useful in manipulating the functions of biological molecules. Here, we describe the first logical circuit based on DNA-protein interactions with accurate threshold control, enabling autonomous, self-sustained and programmable manipulation of protein activity in vitro. Similar circuits made previously were based primarily on DNA hybridization and strand displacement reactions. This new design uses the diverse nucleic acid interactions with proteins. The circuit can precisely sense the local enzymatic environment, such as the concentration of thrombin, and when it is excessively high, a coagulation inhibitor is automatically released by a concentration-adjusted circuit module. To demonstrate the programmable and autonomous modulation, a molecular circuit with different threshold concentrations of thrombin was tested as a proof of principle. In the future, owing to tunable regulation, design modularity and target specificity, this prototype could lead to the development of novel DNA biochemical circuits to control the delivery of aptamer-based drugs in smart and personalized medicine, providing a more efficient and safer therapeutic strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.