ArticleScientific reports2012
Transcription activator-like effector hybrids for conditional control and rewiring of chromosomal transgene expression.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 66 citations in OpenAlex.
- Mammalian synthetic gene circuits for biopharmaceutical development & manufacture.NPJ systems biology and applications · 2025Review
- Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals.International journal of molecular sciences · 2024Review
- Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors.Plant methods · 2022Article
- Cell morphology-based machine learning models for human cell state classification.NPJ systems biology and applications · 2021Article
- Recent developments and applications of genetic transformation and genome editing technologies in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas.FEMS microbiology reviews · 2020Review
- Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta.Plant physiology · 2019Article
- Functional instability allows access to DNA in longer transcription Activator-Like effector (TALE) arrays.eLife · 2019Article
- Article
- Broken TALEs: Transcription Activator-like Effectors Populate Partly Folded States.Biophysical journal · 2016Article
- Genome-Editing Technologies: Principles and Applications.Cold Spring Harbor perspectives in biology · 2016Review
- An engineered tale-transcription factor rescues transcription of factor VII impaired by promoter mutations and enhances its endogenous expression in hepatocytes.Scientific reports · 2016Article
- A transcription activator-like effector (TALE) induction system mediated by proteolysis.Nature chemical biology · 2016Article
- Locked and proteolysis-based transcription activator-like effector (TALE) regulation.Nucleic acids research · 2016Article
- Genome Engineering with TALE and CRISPR Systems in Neuroscience.Frontiers in genetics · 2016Review
- Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions.PloS one · 2016Article
- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Reconfigurable hybrid interface for molecular marker diagnostics and in-situ reporting.Biosensors & bioelectronics · 2015Article
- Discriminating direct and indirect connectivities in biological networks.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Enhanced MyoD-induced transdifferentiation to a myogenic lineage by fusion to a potent transactivation domain.ACS synthetic biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to conditionally rewire pathways in human cells holds great therapeutic potential. Transcription activator-like effectors (TALEs) are a class of naturally occurring specific DNA binding proteins that can be used to introduce targeted genome modifications or control gene expression. Here we present TALE hybrids engineered to respond to endogenous signals and capable of controlling transgenes by applying a predetermined and tunable action at the single-cell level. Specifically, we first demonstrate that combinations of TALEs can be used to modulate the expression of stably integrated genes in kidney cells. We then introduce a general purpose two-hybrid approach that can be customized to regulate the function of any TALE either using effector molecules or a heterodimerization reaction. Finally, we demonstrate the successful interface of TALEs to specific endogenous signals, namely hypoxia signaling and microRNAs, essentially closing the loop between cellular information and chromosomal transgene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.