ArticleNucleic acids research2013
Multiple RNA interactions position Mrd1 at the site of the small subunit pseudoknot within the 90S pre-ribosome.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 18 citations in OpenAlex.
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Conformational switches control early maturation of the eukaryotic small ribosomal subunit.eLife · 2019Article
- Assembly of the small ribosomal subunit in yeast: mechanism and regulation.RNA (New York, N.Y.) · 2018Review
- Maturation of the 90S pre-ribosome requires Mrd1 dependent U3 snoRNA and 35S pre-rRNA structural rearrangements.Nucleic acids research · 2018Article
- 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.Nature structural & molecular biology · 2017Article
- High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast.Nature communications · 2017Article
- Nop9 binds the central pseudoknot region of 18S rRNA.Nucleic acids research · 2017Article
- Article
- Stepwise and dynamic assembly of the earliest precursors of small ribosomal subunits in yeast.Genes & development · 2016Article
- Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.Nature structural & molecular biology · 2015Article
- Article
- Interaction between ribosome assembly factors Krr1 and Faf1 is essential for formation of small ribosomal subunit in yeast.The Journal of biological chemistry · 2014Article
- A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase.RNA (New York, N.Y.) · 2014Article
- Article
- Identification of the binding site of Rlp7 on assembling 60S ribosomal subunits in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2013Article
- Yeast ribosomal protein L7 and its homologue Rlp7 are simultaneously present at distinct sites on pre-60S ribosomal particles.Nucleic acids research · 2013Article
- An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme.PLoS biology · 2013Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Ribosomal subunit biogenesis in eukaryotes is a complex multistep process. Mrd1 is an essential and conserved small (40S) ribosomal subunit synthesis factor that is required for early cleavages in the 35S pre-ribosomal RNA (rRNA). Yeast Mrd1 contains five RNA-binding domains (RBDs), all of which are necessary for optimal function of the protein. Proteomic data showed that Mrd1 is part of the early pre-ribosomal complexes, and deletion of individual RBDs perturbs the pre-ribosomal structure. In vivo ultraviolet cross-linking showed that Mrd1 binds to the pre-rRNA at two sites within the 18S region, in helix 27 (h27) and helix 28. The major binding site lies in h27, and mutational analyses shows that this interaction requires the RBD1-3 region of Mrd1. RBD2 plays the dominant role in h27 binding, but other RBDs also contribute directly. h27 and helix 28 are located close to the sequences that form the central pseudoknot, a key structural feature of the mature 40S subunit. We speculate that the modular structure of Mrd1 coordinates pseudoknot formation with pre-rRNA processing and subunit assembly.
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