ArticleThe Journal of pharmacology and experimental therapeutics2013
Immunodetection of serum albumin adducts as biomarkers for organophosphorus exposure.
Article in The Journal of pharmacology and experimental therapeutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Progressive expansion of albumin adducts for organophosphorus nerve agent traceability based on single and group adduct collection.Analytical and bioanalytical chemistry · 2024Article
- Evidence of nerve agent VX exposure in rat plasma by detection of albumin-adducts in vitro and in vivo.Archives of toxicology · 2023Article
- Retrospective detection for V-type OPNAs exposure via phosphonylation and disulfide adducts in albumin.Scientific reports · 2022Article
- Screening of monoclonal antibodies against specific phosphonylation sites and analysis of serum samples exposed to soman and VX using an indirect competitive enzyme-linked immunosorbent assay.Analytical and bioanalytical chemistry · 2022Article
- Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity.The Journal of biological chemistry · 2018Article
- Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins and Peptides Independent of Surrounding Amino Acids.Chemical research in toxicology · 2017Article
- Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure.Chemical research in toxicology · 2017Article
- PHOS-select iron affinity beads enrich peptides for the detection of organophosphorus adducts on albumin.Chemical research in toxicology · 2013Article
- Polyclonal antibody to soman-tyrosine.Chemical research in toxicology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A major challenge in organophosphate (OP) research has been the identification and utilization of reliable biomarkers for the rapid, sensitive, and efficient detection of OP exposure. Although Tyr 411 OP adducts to human serum albumin (HSA) have been suggested to be one of the most robust biomarkers in the detection of OP exposure, the analysis of HSA-OP adduct detection has been limited to techniques using mass spectrometry. Herein, we describe the procurement of two monoclonal antibodies (mAb-HSA-GD and mAb-HSA-VX) that recognized the HSA Tyr 411 adduct of soman (GD) or S-[2-(diisopropylamino)ethyl]-O-ethyl methylphosphonothioate (VX), respectively, but did not recognize nonphosphonylated HSA. We showed that mAb-HSA-GD was able to detect the HSA Tyr 411 OP adduct at a low level (i.e., human blood plasma treated with 180 nM GD) that could not be detected by mass spectrometry. mAb-HSA-GD and mAb-HSA-VX showed an extremely low-level detection of GD adducted to HSA (on the order of picograms). mAb-HSA-GD could also detect serum albumin OP adducts in blood plasma samples from different animals administered GD, including rats, guinea pigs, and monkeys. The ability of the two antibodies to selectively recognize nerve agents adducted to serum albumin suggests that these antibodies could be used to identify biomarkers of OP exposure and provide a new biologic approach to detect OP exposure in animals.
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Registered trials
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