ArticleBMC infectious diseases2012
Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.
Article in BMC infectious diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Mechanism by which porcine transmissible gastroenteritis virus disrupts host innate immunity.Frontiers in immunology · 2025Review
- Gasdermin E is required for induction of pyroptosis and severe disease during enterovirus 71 infection.The Journal of biological chemistry · 2022Article
- Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India.Scientific reports · 2021Article
- IKKε isoform switching governs the immune response against EV71 infection.Communications biology · 2021Article
- Caspase-3 inhibitor inhibits enterovirus D68 production.Journal of microbiology (Seoul, Korea) · 2020Article
- Senecavirus A 3C Protease Mediates Host Cell Apoptosis Late in Infection.Frontiers in immunology · 2019Article
- EV71 3C protease induces apoptosis by cleavage of hnRNP A1 to promote apaf-1 translation.PloS one · 2019Article
- Antiviral and Inflammatory Cellular Signaling Associated with Enterovirus 71 Infection.Viruses · 2018Review
- Caspase-3 Inhibition Attenuates the Cytopathic Effects of EV71 Infection.Frontiers in microbiology · 2018Article
- Involvement of inducible nitric oxide synthase and mitochondrial dysfunction in the pathogenesis of enterovirus 71 infection.Oncotarget · 2017Article
- AIM2 Inflammasome-Mediated Pyroptosis in Enterovirus A71-Infected Neuronal Cells Restricts Viral Replication.Scientific reports · 2017Article
- Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells.Oncology letters · 2017Article
- Enterovirus 71 infection of motor neuron-like NSC-34 cells undergoes a non-lytic exit pathway.Scientific reports · 2016Article
- JNK1 Derived from Orange-Spotted Grouper, Epinephelus coioides, Involving in the Evasion and Infection of Singapore Grouper Iridovirus (SGIV).Frontiers in microbiology · 2016Article
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- Whether Alzheimer's diseases related genes also differently express in the hippocampus of Ts65Dn mice?International journal of clinical and experimental pathology · 2015Article
- Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death.Virulence · 2015Article
- Targeting protein neddylation with an NEDD8-activating enzyme inhibitor MLN4924 induced apoptosis or senescence in human lymphoma cells.Cancer biology & therapy · 2015Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnterovirus 71 (EV71) infection can induce the apoptosis of infected cells. The aim of this study is to explore the effect of EV71 infection on apoptosis mechanisms in virus-infected human rhabdomyosarcoma (RD) cells.
methodsThe apoptosis of RD cells was examined using annexin V-FITC/PI by flow cytometry and cytokines were detected by ELISA. Cellular RNA was extracted and transcribed to cDNA. PCR array was employed to analyze the expressions of 84 apoptotic genes from EV71-infected RD cells at 8 and 20 h postinfection, respectively. In addition, the expressions of FasL, caspase, AKT2, JNK1/2, c-Jun and NF-κB proteins were detected by western blotting.
resultsFlow cytometry demonstrated that the apoptosis or death of EV71-infected RD cells was increased by 37.1% with a multiplicity of infection (MOI) of 5 at 20 h postinfection. The production of IL-4, IL-10 and TNF-α was enhanced by the subsequent EV71 infection. PCR array revealed significant changes in the expressions of apoptotic genes. Among 84 genes, 42 genes were down-regulated after EV71 infection at 8 h, whereas 32 genes were up-regulated at 20 h postinfection. Moreover, the ligands of TNF superfamily such as FasL, CD40L and TNF-α were significantly up-regulated and enhanced the expressions of apoptosis-related cysteine peptidases, including caspase-10, -8, -7 and -3. In addition, EV71 infection induces the phosphorylation of AKT2, JNK1/2, c-Jun and NF-κB at 20 h postinfection.
conclusionPCR array for the determination of apoptosis gene expressions is an informative assay in elucidating biological pathways. During the early stage of EV71 infection, the apoptotic process of RD cells is significantly delayed. EV71 infection can also induce the expressions of FasL, TNF-α and CD40L, which contribute to the apoptosis of RD cells.
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